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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-4-11
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pubmed:abstractText |
The cardiac sodium current was studied in guinea pig ventricular myocytes using the cell-attached patch voltage clamp at 37 degrees C in the presence of 145 mM external sodium concentration. When using large patch pipettes (access resistance, 1-2 M omega), the capacity current transient duration was typically 70 microseconds for voltage clamp steps up to 150 mV. At 37 degrees C the maximum inward sodium current peaked in approximately 200 microseconds after the onset of a clamp step and at this strong depolarization, less than 10% of the sodium current developed during the capacity transient. The sodium current developed smoothly and the descending limb of the current-voltage relationship usually spanned a range of 40 mV. Moreover, currents reduced by inactivation of sodium channels could be scaled to superimpose on the maximum current. Current tails elicited by deactivation followed a monoexponential time course that was very similar for currents of different sizes. Data obtained over a range of temperatures (15 degrees-35 degrees C) showed that the steady-state inactivation and conductance-voltage curves were shifted to more negative voltages at lower temperatures. These results demonstrate the feasibility of investigating the sodium current of mammalian cardiac cells at 37 degrees C in normal physiological solutions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-1182931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-13694548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-238230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2409525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2415670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2435839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2448058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2456164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2484076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2547248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2548634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2561792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-2582086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-423977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-4951054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-6145329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-6289259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-6329543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-7108778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-7177773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-7320902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155034-751104
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
607-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1990
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pubmed:articleTitle |
Voltage clamp of the cardiac sodium current at 37 degrees C in physiologic solutions.
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pubmed:affiliation |
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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