Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-4-11
pubmed:abstractText
Recent evidence suggests that cocaine can produce marked cardiac arrhythmias and sudden death. A possible mechanism for this effect is slowing of impulse conduction due to block of cardiac Na channels. We therefore investigated its effects on Na channels in isolated guinea pig ventricular myocytes using the whole-cell variant of the patch clamp technique. Cocaine (10-50 microM) was found to reduce Na current in a use-dependent manner. The time course for block development and recovery were characterized. At 30 microM cocaine, two phases of block development were defined: a rapid phase (tau = 5.7 +/- 4.9 ms) and a slower phase (tau = 2.3 +/- 0.7 s). Recovery from block at -140 mV was also defined by two phases: (tau f = 136 +/- 61 ms, tau s = 8.5 +/- 1.7 s) (n = 6). To further clarify the molecular mechanisms of cocaine action on cardiac Na channels, we characterized its effects using the guarded receptor model, obtaining estimated Kd values of 328, 19, and 8 microM for channels predominantly in the rested, activated, and inactivated states. These data indicate that cocaine can block cardiac Na channels in a use-dependent manner and provides a possible cellular explanation for its cardiotoxic effects.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-1081138, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-19431557, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-21711, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-233568, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2410043, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2414642, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2415670, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2424514, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2429564, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2443192, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2443658, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2446909, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2553294, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2846199, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2851029, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2966899, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-2998207, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-300786, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-334262, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-3785295, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-4067533, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-439335, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-4541078, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-4541340, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6270629, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6306139, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6316783, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6326044, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6331543, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6360570, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-637436, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6471319, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-6488910, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-687766, http://linkedlifedata.com/resource/pubmed/commentcorrection/2155033-7371135
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
589-99
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Characterization of cocaine-induced block of cardiac sodium channels.
pubmed:affiliation
Department of Pharmacology, School of Medicine, Tulane University, New Orleans, Louisiana 70112.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.