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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2011-8-22
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pubmed:abstractText |
Antigen-immunoglobulin fusion protein expressing B cells have been shown as excellent tolerogenic antigen-presenting cells in multiple disease models. Using efficient protein transduction by fusion with a HIV TAT protein transduction domain, we herein tested the TAT-fusion protein transduced B cells for their effects in antigen-specific tolerance induction in two animal models, experimental autoimmune encephalomyelitis (EAE) and type 1 diabetes. We demonstrated that transfer of TAT-MOG35-55 (myelin oligodendrocyte glycoprotein)-Ig 'transduced B cells' 10 days after EAE induction significantly protected mice from disease. Similarly, the onset of disease was delayed when NOD mice received insulin specific TAT-B9-23-B cells. Surprisingly, no protection against EAE was observed in a prophylactic protocol when transduced B cells were given before disease induction. Moreover, TAT-ovalbumin transduced cells were tolerogenic in primed but not naïve mice. Our results suggest that TAT-fusion protein transduced B cells were tolerogenic in antigen primed recipients, a condition clinically relevant to autoimmune diseases.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1521-7035
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pubmed:author |
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pubmed:copyrightInfo |
Published by Elsevier Inc.
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pubmed:issnType |
Electronic
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pubmed:volume |
140
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
260-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:21546313-Adoptive Transfer,
pubmed-meshheading:21546313-Animals,
pubmed-meshheading:21546313-B-Lymphocytes,
pubmed-meshheading:21546313-Diabetes Mellitus, Type 1,
pubmed-meshheading:21546313-Encephalomyelitis, Autoimmune, Experimental,
pubmed-meshheading:21546313-Female,
pubmed-meshheading:21546313-Gene Products, tat,
pubmed-meshheading:21546313-Immune Tolerance,
pubmed-meshheading:21546313-Insulin,
pubmed-meshheading:21546313-Mice,
pubmed-meshheading:21546313-Mice, Inbred C57BL,
pubmed-meshheading:21546313-Mice, Inbred NOD,
pubmed-meshheading:21546313-Mice, Transgenic,
pubmed-meshheading:21546313-Myelin Proteins,
pubmed-meshheading:21546313-Myelin-Associated Glycoprotein,
pubmed-meshheading:21546313-Peptide Fragments,
pubmed-meshheading:21546313-Recombinant Fusion Proteins
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pubmed:year |
2011
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pubmed:articleTitle |
B cells "transduced" with TAT-fusion proteins can induce tolerance and protect mice from diabetes and EAE.
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pubmed:affiliation |
Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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