Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Pt 1
|
| pubmed:dateCreated |
1990-3-1
|
| pubmed:abstractText |
In rats infused with NaH14CO3, 14CO2 exhalation approached an asymptotic rate equal to 96.0 +/- 1.3 (SD) % of the infusion rate of 14C in a monoexponential manner with a half-time of 20 min. In rats infused with [1-14C]leucine, 14CO2 exhalation (/0.96) approached an asymptotic value, (1-F), of 16-25% of 14C infusion rate (mean 20.4%) in a monoexponential manner with a half-time of approximately 31 min. Whole body protein synthesis (S) was calculated from 1-F and urea N plus ammonia N excretion (C) as S = CF/(1-F). S was a uniform function of body weight in these rats, in six additional rats in which S was measured 6 h after single intravenous injection of [1-14C]leucine and also in previously reported rats given single injections. The relationship was S (mg.100 g-1.h-1) = 11-0.143 body wt (g) +/- 8.3. In six of these rats, S was also estimated from the plateau specific activity of plasma leucine or plasma 2-ketoisocaproate (KIC); the former estimate of S was significantly lower (by an average of 17%), but S was the same when specific activity of KIC was employed. These results support the validity of the expired 14CO2 technique for measuring S.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ammonia,
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Bicarbonate,
http://linkedlifedata.com/resource/pubmed/chemical/Urea
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
258
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
E151-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2154116-Ammonia,
pubmed-meshheading:2154116-Animals,
pubmed-meshheading:2154116-Bicarbonates,
pubmed-meshheading:2154116-Carbon Dioxide,
pubmed-meshheading:2154116-Carbon Radioisotopes,
pubmed-meshheading:2154116-Infusions, Intravenous,
pubmed-meshheading:2154116-Kinetics,
pubmed-meshheading:2154116-Leucine,
pubmed-meshheading:2154116-Male,
pubmed-meshheading:2154116-Oxidation-Reduction,
pubmed-meshheading:2154116-Protein Biosynthesis,
pubmed-meshheading:2154116-Radioisotope Dilution Technique,
pubmed-meshheading:2154116-Rats,
pubmed-meshheading:2154116-Rats, Inbred Strains,
pubmed-meshheading:2154116-Respiration,
pubmed-meshheading:2154116-Sodium,
pubmed-meshheading:2154116-Sodium Bicarbonate,
pubmed-meshheading:2154116-Urea
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Estimation of whole body protein synthesis from oxidation of infused [1-14C]leucine.
|
| pubmed:affiliation |
Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|