Linked Life Data

21539812

Source:http://linkedlifedata.com/resource/pubmed/id/21539812

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-7-4
pubmed:abstractText
Geminin plays an important role in coordinating the cell cycle with anterior-posterior patterning during embryonic development. However, whether it is involved in the regulation of left-right (LR) patterning remains unknown. Here, we reported that geminin is required for setting up heart and visceral laterality during zebrafish development. Defective heart and visceral laterality was observed in geminin morphants. Further study demonstrated that the left-sided nodal/spaw in the lateral plate mesoderm (LPM) as well as the sideness of its downstream targets lefty2 and lefty1 was perturbed in geminin morphants. Upstream of the left-sided Nodal signal along the regulatory cascade of LR asymmetry, knock down of geminin resulted in defective Kupffer's vesicle (KV) formation and ciliogenesis rather than middle line defects. Predominant distribution of an antisense morpholino against geminin in dorsal forerunner cells (DFCs) led to defective KV morphogenesis and perturbed LR asymmetry, similar to those of geminin morphants, indicating a cell-autonomous role of geminin in regulating KV formation and ciliogenesis. Our results demonstrate that geminin is required for proper KV formation and ciliogenesis, thus playing an important part in setting up LR asymmetry.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1090-2104
pubmed:author
pubmed:copyrightInfo
Copyright © 2011. Published by Elsevier Inc.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
410
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
164-9
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Geminin is required for left-right patterning through regulating Kupffer's vesicle formation and ciliogenesis in zebrafish.
pubmed:affiliation
Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Chongqing 400715, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't