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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-3-15
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pubmed:abstractText |
To investigate the regulation of membrane voltage and transmembrane ion fluxes in human neutrophils, we studied plasma membrane currents using the whole-cell patch-clamp method. We observed three distinct ion channel currents: (a) a voltage-dependent K+ current, (b) a Ca2(+)-activated K+ current, and (c) a Ca2(+)-activated Cl- current. The voltage-dependent K+ current was found in cells at rest. Its conductive properties suggested an inwardly rectifying channel. The channel was activated at membrane potentials more positive than -60 mV, suggesting that it may determine the resting membrane potential of neutrophils. Activation of neutrophils by the Ca2+ ionophore ionomycin led to an increase in whole-cell K+ and Cl- currents. The Ca2(+)-activated K+ channel differed from the voltage-dependent K+ channel because it was insensitive to voltage, because it rectified outwardly, and because the voltage-sensitive K+ channel was Ca2(+)-independent. The Ca2(+)-activated Cl- channel showed outward rectification and no apparent voltage dependency. The Ca2(+)-activated K+ and Cl- channels may play a role in cell volume homeostasis and/or cellular activation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2408670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2419551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2431318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2435712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2442173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2444891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-278994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2825632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2836507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2838910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-2925648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-3072727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-3077552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-3543977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-3746250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-3877077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-6097318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-6296371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-6327398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-6328495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-6770097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-7159582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-7175489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153708-7291986
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
491-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1990
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pubmed:articleTitle |
Voltage-dependent and Ca2(+)-activated ion channels in human neutrophils.
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pubmed:affiliation |
Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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