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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-7-18
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pubmed:abstractText |
The GABA(C) receptor, a postsynaptic membrane receptor expressed prominently in the retina, is a ligand-gated ion channel that consists of a combination of ? subunits. We report characterization of a novel guinea pig polyclonal antibody, termed GABA(C) Ab N-14, directed against a 14-mer peptide (N-14) of the extracellular domain of the human ?1 subunit. The antibody exhibits high sensitivity for N-14 by ELISA. In Western blots, GABA(C) Ab N-14 shows reactivity with the ?1 subunit of preparations obtained from ?1 GABA(C)-expressing neuroblastoma cells, Xenopus oocytes, and mammalian retina and brain. Flow cytometry reveals a rightward shift in mean fluorescence intensity of GABA(C)-expressing neuroblastoma cells probed with GABA(C) Ab N-14. Immunostaining of neuroblastoma cells and oocytes with GABA(C) Ab N-14 yields fluorescence only with GABA(C)-expressing cells. Antibody binding has no effect on GABA-elicited membrane current responses. Immunostaining of human retinal sections shows preferential staining within the inner plexiform layer. GABA(C) Ab N-14 appears well suited for investigative studies of GABA(C) ?1 subunit in retina and other neural tissues.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/EY001792,
http://linkedlifedata.com/resource/pubmed/grant/EY016094,
http://linkedlifedata.com/resource/pubmed/grant/HL24530,
http://linkedlifedata.com/resource/pubmed/grant/P30 EY001792-30,
http://linkedlifedata.com/resource/pubmed/grant/P30 EY001792-30S1,
http://linkedlifedata.com/resource/pubmed/grant/P30 EY001792-31A1,
http://linkedlifedata.com/resource/pubmed/grant/P30 EY001792-32,
http://linkedlifedata.com/resource/pubmed/grant/P30 EY001792-33,
http://linkedlifedata.com/resource/pubmed/grant/R01 EY016094-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 EY016094-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 EY016094-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 EY016094-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 EY016094-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 HL024530-24,
http://linkedlifedata.com/resource/pubmed/grant/R01 HL024530-25A2,
http://linkedlifedata.com/resource/pubmed/grant/R01 HL024530-26,
http://linkedlifedata.com/resource/pubmed/grant/R01 HL024530-27,
http://linkedlifedata.com/resource/pubmed/grant/R01 HL024530-28
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1096-0007
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-64
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:21536029-Amino Acid Sequence,
pubmed-meshheading:21536029-Animals,
pubmed-meshheading:21536029-Antibodies,
pubmed-meshheading:21536029-Antibody Specificity,
pubmed-meshheading:21536029-Blotting, Western,
pubmed-meshheading:21536029-Brain,
pubmed-meshheading:21536029-Chromatography, Affinity,
pubmed-meshheading:21536029-Cross Reactions,
pubmed-meshheading:21536029-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:21536029-Flow Cytometry,
pubmed-meshheading:21536029-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:21536029-Guinea Pigs,
pubmed-meshheading:21536029-Humans,
pubmed-meshheading:21536029-Immunoglobulin G,
pubmed-meshheading:21536029-Molecular Sequence Data,
pubmed-meshheading:21536029-Neuroblastoma,
pubmed-meshheading:21536029-Oocytes,
pubmed-meshheading:21536029-Peptide Fragments,
pubmed-meshheading:21536029-Protein Subunits,
pubmed-meshheading:21536029-Receptors, GABA,
pubmed-meshheading:21536029-Retina,
pubmed-meshheading:21536029-Tumor Cells, Cultured,
pubmed-meshheading:21536029-Xenopus laevis
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pubmed:year |
2011
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pubmed:articleTitle |
Subunit-specific polyclonal antibody targeting human ?1 GABA(C) receptor.
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pubmed:affiliation |
Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, 1855 W. Taylor St., Chicago, IL 60612, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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