Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-5-2
pubmed:abstractText
Lysophosphatidic acid (LPA) is an agonist for peroxisome proliferator activated receptor-? (PPAR?). Although glycerol-3-phosphate acyltransferase-1 (GPAT1) esterifies glycerol-3-phosphate to form LPA, an intermediate in the de novo synthesis of glycerolipids, it has been assumed that LPA synthesized by this route does not have a signaling role. The availability of Chinese Hamster Ovary (CHO) cells that stably overexpress GPAT1, allowed us to analyze PPAR? activation in the presence of LPA produced as an intracellular intermediate. LPA levels in CHO-GPAT1 cells were 6-fold higher than in wild-type CHO cells, and the mRNA abundance of CD36, a PPAR? target, was 2-fold higher. Transactivation assays showed that PPAR? activity was higher in the cells that overexpressed GPAT1. PPAR? activity was enhanced further in CHO-GPAT1 cells treated with the PPAR? ligand troglitazone. Extracellular LPA, phosphatidic acid (PA) or a membrane-permeable diacylglycerol had no effect, showing that PPAR? had been activated by LPA generated intracellularly. Transient transfection of a vector expressing 1-acylglycerol-3-phosphate acyltransferase-2, which converts endogenous LPA to PA, markedly reduced PPAR? activity, as did over-expressing diacylglycerol kinase, which converts DAG to PA, indicating that PA could be a potent inhibitor of PPAR?. These data suggest that LPA synthesized via the glycerol-3-phosphate pathway can activate PPAR? and that intermediates of de novo glycerolipid synthesis regulate gene expression.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-10918059, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-11089532, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-11546763, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-11818483, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-11956205, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-12006582, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-12208858, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-12354767, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-12502787, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-14559069, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-14654091, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-14994966, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-15007093, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-15023998, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-15271293, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-15273989, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-15629135, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-15780969, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-15878874, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-16254047, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-16263710, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-16321982, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-16574237, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-17389595, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-18658143, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-20200319, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-20705243, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-2981862, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-3968089, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-7684926, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-7978274, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-9113987, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-9514791, http://linkedlifedata.com/resource/pubmed/commentcorrection/21533082-9556573
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1932-6203
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e18932
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Lysophosphatidic acid activates peroxisome proliferator activated receptor-? in CHO cells that over-express glycerol 3-phosphate acyltransferase-1.
pubmed:affiliation
Department of Nutrition, University of North Carolina, Chapel Hill, North Carolina, United States of America.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural