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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-2-9
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pubmed:databankReference |
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pubmed:abstractText |
The baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV, which is representative of the MNPV subtype in which the virions may contain many nucleocapsids within a single viral envelope) encodes a protein, v-ubi, that has 76% identity with the eukaryotic protein ubiquitin. Transcriptional mapping indicated that the gene for v-ubi was transcribed during the late phase of viral infection. Two transcriptional start sites potentially encoding v-ubi were identified. Both sites were contained within a sequence motif common to baculovirus late genes. A recombinant virus, AcUbi-beta Gal, encoding a ubiquitin-beta-galactosidase fusion protein was constructed to monitor the temporal regulation of v-ubi gene during viral infection. The fusion protein was expressed maximally at 14-18 hr postinfection, consistent with its classification as a late protein. The amount of ubiquitin-beta-galactosidase fusion protein that accumulated in AcUbi-beta Gal-infected cells by 48 hr postinfection was approximately 14% of the level of beta-galactosidase that was synthesized under control of the polyhedrin promoter. Transcriptional analysis confirmed that synthesis of the fusion protein was directed by the v-ubi gene promoter. AcUbi-beta Gal also produced normal levels of authentic viral ubiquitin message. Southern blot analysis of AcUbi-beta Gal and 15 additional isolates revealed that the fusion sequences had not recombined at the ubiquitin locus. A polyubiquitin gene was isolated and sequenced from Spodoptera frugiperda, a lepidopteran host cell line for AcMNPV. The predicted amino acid sequence of the product of the host gene is identical to animal ubiquitin.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2440890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2463465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2494430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2497580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2538753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2538756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2538923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2822254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2825030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2829204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2838968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2887077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-2987683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-3003914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-3018930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-3038523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-3292791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-3525744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-3944847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-6319233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2153300-6546423
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
409-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2153300-Amino Acid Sequence,
pubmed-meshheading:2153300-Animals,
pubmed-meshheading:2153300-Base Sequence,
pubmed-meshheading:2153300-Cell Line,
pubmed-meshheading:2153300-DNA, Viral,
pubmed-meshheading:2153300-Genes, Viral,
pubmed-meshheading:2153300-Humans,
pubmed-meshheading:2153300-Insect Viruses,
pubmed-meshheading:2153300-Molecular Sequence Data,
pubmed-meshheading:2153300-Moths,
pubmed-meshheading:2153300-Restriction Mapping,
pubmed-meshheading:2153300-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2153300-Ubiquitins,
pubmed-meshheading:2153300-Viral Proteins,
pubmed-meshheading:2153300-Viral Structural Proteins,
pubmed-meshheading:2153300-Virion
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pubmed:year |
1990
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pubmed:articleTitle |
Identification of a viral gene encoding a ubiquitin-like protein.
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pubmed:affiliation |
Department of Entomology, Texas Agricultural Experiment Station, College Station 77843.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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