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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1990-2-5
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pubmed:abstractText |
A major protein of Mr 68,000 with a previously described protein kinase activity (PK68), which is induced in HCMV-infected cells, is shown to be virus encoded by means of hybrid selection. The gene coding for the corresponding 4-kb mRNA was located within map units 0.510 and 0.525. This location is the same as that of pp65, a structural protein with no attributed function. The early nature of the protein was confirmed with its mRNA first appearing 3 hr after infection under cycloheximide reversal and phosphonoacetic acid blocking conditions. Another mRNA, 97 nucleotides smaller and 5' coterminal with PK68, which codes for a Mr 52,000 protein, was also observed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0042-6822
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
276-85
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2152994-Blotting, Northern,
pubmed-meshheading:2152994-Cell Line,
pubmed-meshheading:2152994-Cloning, Molecular,
pubmed-meshheading:2152994-Cytomegalovirus,
pubmed-meshheading:2152994-Genes, Viral,
pubmed-meshheading:2152994-Humans,
pubmed-meshheading:2152994-Nucleic Acid Hybridization,
pubmed-meshheading:2152994-Precipitin Tests,
pubmed-meshheading:2152994-Protein Biosynthesis,
pubmed-meshheading:2152994-Protein Kinases,
pubmed-meshheading:2152994-RNA, Messenger,
pubmed-meshheading:2152994-RNA, Viral,
pubmed-meshheading:2152994-Restriction Mapping,
pubmed-meshheading:2152994-Transcription, Genetic,
pubmed-meshheading:2152994-Viral Structural Proteins
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pubmed:year |
1990
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pubmed:articleTitle |
Genomic location of a human cytomegalovirus protein with protein kinase activity (PK68).
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pubmed:affiliation |
Unité de Virologie Médicale, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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