Linked Life Data

21529380

Source:http://linkedlifedata.com/resource/pubmed/id/21529380

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-6-14
pubmed:abstractText
The airway epithelium forms a barrier against infection but also produces antimicrobial peptides (AMPs) and other inflammatory mediators to activate the immune system. It has been shown that in allergic disorders, Th2 cytokines may hamper the antimicrobial activity of the epithelium. However, the presence of Th2 cytokines also affects the composition of the epithelial layer which may alter its function. Therefore, we investigated whether exposure of human primary bronchial epithelial cells (PBEC) to Th2 cytokines during mucociliary differentiation affects expression of the human cathelicidin antimicrobial protein (hCAP18)/LL-37 and human beta defensins (hBD), and antimicrobial activity.PBEC were cultured at an air-liquid interface (ALI) for two weeks in the presence of various concentrations of IL-4 or IL-13. Changes in differentiation and in expression of various AMPs and the antimicrobial proteinase inhibitors secretory leukocyte protease inhibitor (SLPI) and elafin were investigated as well as antimicrobial activity.IL-4 and IL-13 increased mRNA expression of hCAP18/LL-37 and hBD-2. Dot blot analysis also showed an increase in hCAP18/LL-37 protein in apical washes of IL-4-treated ALI cultures, whereas Western Blot analysis showed expression of a protein of approximately 4.5 kDa in basal medium of IL-4-treated cultures. Using sandwich ELISA we found that also hBD-2 in apical washes was increased by both IL-4 and IL-13. SLPI and elafin levels were not affected by IL-4 or IL-13 at the mRNA or protein level. Apical wash obtained from IL-4- and IL-13-treated cultures displayed increased antimicrobial activity against Pseudomonas aeruginosa compared to medium-treated cultures. In addition, differentiation in the presence of Th2 cytokines resulted in increased MUC5AC production as has been shown previously.These data suggest that prolonged exposure to Th2 cytokines during mucociliary differentiation contributes to antimicrobial defence by increasing the expression and release of selected antimicrobial peptides and mucus.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-10079098, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-10645890, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-10837369, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-10979241, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-11053013, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-11466403, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-11748265, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-11796631, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-12023836, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-12374875, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-12594054, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-12794003, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-12960356, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-14707090, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-14978112, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-15020299, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-15034083, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-15242845, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-1530827, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-16051067, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-16546102, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-16740309, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-16849494, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-16963160, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-17215317, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-17216197, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-17617590, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-20094876, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-8100770, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-8534481, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-8890201, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-9242546, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-9689116, http://linkedlifedata.com/resource/pubmed/commentcorrection/21529380-9856807
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antimicrobial Cationic Peptides, http://linkedlifedata.com/resource/pubmed/chemical/Cathelicidins, http://linkedlifedata.com/resource/pubmed/chemical/DEFB4A protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Elafin, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4, http://linkedlifedata.com/resource/pubmed/chemical/MUC5AC protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Mucin 5AC, http://linkedlifedata.com/resource/pubmed/chemical/PI3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/SLPI protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Secretory Leukocyte Peptidase..., http://linkedlifedata.com/resource/pubmed/chemical/beta-Defensins, http://linkedlifedata.com/resource/pubmed/chemical/beta-defensin 3, human, http://linkedlifedata.com/resource/pubmed/chemical/cathelicidin antimicrobial peptide
pubmed:status
MEDLINE
pubmed:issn
1465-993X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59
pubmed:meshHeading
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