Linked Life Data

21525380

Source:http://linkedlifedata.com/resource/pubmed/id/21525380

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2011-5-20
pubmed:abstractText
Stimulation of naive mouse CD4(+)Foxp3(-) T cells in the presence of TGF-? results in the induction of Foxp3 expression and T suppressor function. However, Foxp3 expression in these induced regulatory T cells (iTreg) is unstable, raising the possibility that iTreg would not be useful for treatment of autoimmune diseases. To analyze the factors that control the stability of Foxp3 expression in iTreg, we generated OVA-specific iTreg from OT-II Foxp3-GFP knockin mice. Following transfer to normal C57BL/6 mice, OT-II GFP(+) cells maintained high levels of Foxp3 expression for 8 d. However, they rapidly lost Foxp3 expression upon stimulation with OVA in IFA in vivo. This unstable phenotype was associated with a strong methylation of the Treg-specific demethylated region within the Foxp3 locus. Administration of IL-2/anti-IL-2 complexes expanded the numbers of transferred Foxp3(+) iTreg in the absence of Ag challenge. Notably, when the iTreg were stimulated with Ag, treatment with IL-2/anti-IL-2 complexes stabilized Foxp3 expression and resulted in enhanced demethylation of the Treg-specific demethylated region. Conversely, neutralization of IL-2 or disruption of its signaling by deletion of Stat5 diminished the level of Foxp3 expression resulting in decreased suppressor function of the iTreg in vivo. Our data suggest that stimulation with TGF-? in vitro is not sufficient for imprinting T cells with stable expression of Foxp3. Administration of IL-2 in vivo results in stabilization of Foxp3 expression and may prove to be a valuable adjunct for the use of iTreg for the treatment of autoimmune diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
186
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6329-37
pubmed:meshHeading
pubmed-meshheading:21525380-Adoptive Transfer, pubmed-meshheading:21525380-Animals, pubmed-meshheading:21525380-Antibodies, pubmed-meshheading:21525380-Cells, Cultured, pubmed-meshheading:21525380-DNA Methylation, pubmed-meshheading:21525380-Flow Cytometry, pubmed-meshheading:21525380-Forkhead Transcription Factors, pubmed-meshheading:21525380-Green Fluorescent Proteins, pubmed-meshheading:21525380-Interleukin-2, pubmed-meshheading:21525380-Male, pubmed-meshheading:21525380-Mice, pubmed-meshheading:21525380-Mice, Inbred C57BL, pubmed-meshheading:21525380-Mice, Knockout, pubmed-meshheading:21525380-Mice, Transgenic, pubmed-meshheading:21525380-Ovalbumin, pubmed-meshheading:21525380-Promoter Regions, Genetic, pubmed-meshheading:21525380-Receptors, Antigen, T-Cell, pubmed-meshheading:21525380-Recombinant Fusion Proteins, pubmed-meshheading:21525380-STAT5 Transcription Factor, pubmed-meshheading:21525380-Signal Transduction, pubmed-meshheading:21525380-T-Lymphocytes, pubmed-meshheading:21525380-T-Lymphocytes, Regulatory, pubmed-meshheading:21525380-Transforming Growth Factor beta
pubmed:year
2011
pubmed:articleTitle
IL-2 controls the stability of Foxp3 expression in TGF-beta-induced Foxp3+ T cells in vivo.
pubmed:affiliation
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Intramural