Linked Life Data

21524506

Source:http://linkedlifedata.com/resource/pubmed/id/21524506

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-4-28
pubmed:abstractText
?-Lactalbumin is a ubiquitous calcium-binding milk protein with a well-characterized function in regulating the synthesis of lactose. An entirely different activity has been shown to occur when a complex is formed between calcium-free ?-lactalbumin and oleic acid. This complex shows strong cytotoxic action against several cancer cells, and several mechanisms have been suggested to account for this cell-killing activity. Most studies have been performed using the human protein, but bovine ?-lactalbumin shows similar activity. A new and simple 2-step method for purification of calcium-free ?-lactalbumin has been developed, and the resulting highly purified preparation was used to generate a complex with oleic acid. Using 3 different cell lines and 2 types of cell viability assays, the bovine and human ?-lactalbumin showed comparable cytotoxic activity. The effect was apparent after 15 min of incubation and was inhibited by the presence of fetal bovine serum or bovine serum albumin. The bovine protein might be a useful alternative to the human protein, but also raises the question whether cytotoxic activity could be generated in different kinds of food containing ?-lactalbumin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1525-3198
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2159-70
pubmed:dateRevised
2011-8-24
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human ?-lactalbumin.
pubmed:affiliation
The Protein Chemistry Laboratory, Department of Molecular Biology, Aarhus University, Science Park, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark.
pubmed:publicationType
Journal Article, Comparative Study