Linked Life Data

21515364

Source:http://linkedlifedata.com/resource/pubmed/id/21515364

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-6-6
pubmed:abstractText
Cells of the carotenoidless strain R-26.1 of Rhodobacter sphaeroides were grown in the presence of a high concentration (5 mM) of cobalt ions. The photosynthetic intracytoplasmic membranes were isolated and investigated by proteomic analysis using non-denaturating blue native electrophoresis in combination with LC-ESI-MS/MS. Comparison with intracytoplasmic membranes of cells grown under control conditions showed a change in the relative amount of proteins belonging to the photosynthetic apparatus, with net downregulation of light-harvesting complexes and increased concentration of the nude reaction center (RC), as well as upregulation of enzymes related to chemoorganotrophy. These effects represent possible bacterial adaptation so as to retrieve energy for metabolic processes from sources alternative to less efficient photosynthesis. The influence of cobalt on the photochemistry of the RC in cell extracts was also investigated by charge recombination. The kinetics of the charge recombination reaction was found to be slower in extracts from cells exposed to Co(2+), indicating that the reorganization of the photosynthetic apparatus also involves its photochemical core.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1769-7123
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Institut Pasteur. Published by Elsevier SAS. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
520-7
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
The photosynthetic membrane proteome of Rhodobacter sphaeroides R-26.1 exposed to cobalt.
pubmed:affiliation
Italian National Research Council, Institute for Chemical-Physical Processes, Via Orabona 4, 70124 Bari, Italy.
pubmed:publicationType
Journal Article