Source:http://linkedlifedata.com/resource/pubmed/id/21511408
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
2011-7-15
|
| pubmed:abstractText |
This study used PCR-RFLP to investigate the genetic variability of pmp-encoding genes from fifty-two Chlamydophila abortus (C. abortus) strains originating from abortion cases from various geographical regions and host species. Six primer pairs were used to PCR-amplify DNA fragments encoding eighteen pmps. PCR products were digested using four restriction endonucleases and Bayesian methodologies were used to compare RFLP profiles and assign strains to a RFLP genotype. Strains could be assigned to 2 genotypes in the region encoding pmp18D, 3 genotypes in the regions encoding pmp1A-pmp2B, pmp3E-pmp6H and pmp11G-pmp15G, 4 genotypes in the region encoding pmp7G-pmp10G and 5 genotypes in the region encoding pmp16G-pmp17G. In all regions, the majority of strains (88.4-96.1%) had the same genotype as the reference strain S26/3. No correlation could be made between genotype, host species or geographical origin except for the two variant Greek strains, LLG and POS, which formed a discrete genotype in all pmp-encoding regions except pmp18D. Relative rates of evolution calculated for each pmp-encoding gene locus suggest that differing selective pressures and functional constraints may exist on C. abortus polymorphic membrane proteins. These findings suggest that although intraspecies heterogeneity of pmp-encoding genes in C. abortus is low, the sequence heterogeneity should be an important consideration when using pmps as the basis for novel diagnostics or vaccine development.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1873-2542
|
| pubmed:author |
pubmed-author:CaroMaría RMR,
pubmed-author:ClarkEwan MEM,
pubmed-author:LainsonF AlexFA,
pubmed-author:LivingstoneMoragM,
pubmed-author:LongbottomDavidD,
pubmed-author:MagninoSimoneS,
pubmed-author:MarkeyBryan KBK,
pubmed-author:SachseKonradK,
pubmed-author:SaitMichelleM,
pubmed-author:SiarkouVictoria IVI,
pubmed-author:SmithDavid G EDG,
pubmed-author:SpaldingLucyL,
pubmed-author:VretouEvangeliaE,
pubmed-author:WheelhouseNicholasN,
pubmed-author:WrightFrankF,
pubmed-author:YagaRajaR
|
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier B.V. All rights reserved.
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
5
|
| pubmed:volume |
151
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
284-90
|
| pubmed:meshHeading |
pubmed-meshheading:21511408-Abortion, Veterinary,
pubmed-meshheading:21511408-Animals,
pubmed-meshheading:21511408-Bacterial Outer Membrane Proteins,
pubmed-meshheading:21511408-Base Sequence,
pubmed-meshheading:21511408-Bayes Theorem,
pubmed-meshheading:21511408-Chlamydophila,
pubmed-meshheading:21511408-Chlamydophila Infections,
pubmed-meshheading:21511408-DNA Primers,
pubmed-meshheading:21511408-Female,
pubmed-meshheading:21511408-Genetic Variation,
pubmed-meshheading:21511408-Genotype,
pubmed-meshheading:21511408-Geography,
pubmed-meshheading:21511408-Livestock,
pubmed-meshheading:21511408-Polymerase Chain Reaction,
pubmed-meshheading:21511408-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:21511408-Pregnancy
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes.
|
| pubmed:affiliation |
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK. michelle.sait@moredun.ac.uk
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|