Linked Life Data

2150922

Source:http://linkedlifedata.com/resource/pubmed/id/2150922

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-5-29
pubmed:abstractText
Certain T cell ligands can stimulate most or all T cells whose receptor is encoded by particular V beta genes. Exposure to these same ligands during intrathymic development leads to deletion of T cells bearing these same receptors. We have utilized one such ligand, staphylococcal enterotoxin B (SEB), to examine the quantitative differences in these two responses. By comparing the dose of SEB required to delete developing T cells in thymic organ culture with that required to stimulate spleen cells to expand clonally, we observe that clonal deletion is one to two orders of magnitude more sensitive to SEB. Because the T cell ligand involves not only SEB but also class II MHC molecules, and because the culture conditions are distinct, this system does not allow one to state that intrathymic T cells are intrinsically more sensitive to negative selection than peripheral T cells are to activation. However, these studies for the first time do establish a quantitative comparison of these two processes and suggest that there is a margin for error built into the clonal deletion process, such that ligands presented in the thymus are 30- to 100-fold more active in clonal deletion than is the same ligand in activation of peripheral T cells. This result agrees well with the observation that naturally occurring unknown ligands associated with I-E can clonally delete cells that are not activated by the same ligand in mixed lymphocyte culture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:geneSymbol
Mls-2<up>a</up>, V<down>;bgr</down>8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2150922-Animals, pubmed-meshheading:2150922-Antigens, Surface, pubmed-meshheading:2150922-Cell Differentiation, pubmed-meshheading:2150922-Clone Cells, pubmed-meshheading:2150922-Dose-Response Relationship, Drug, pubmed-meshheading:2150922-Enterotoxins, pubmed-meshheading:2150922-Histocompatibility Antigens Class II, pubmed-meshheading:2150922-Ligands, pubmed-meshheading:2150922-Lymphocyte Activation, pubmed-meshheading:2150922-Mice, pubmed-meshheading:2150922-Mice, Inbred BALB C, pubmed-meshheading:2150922-Minor Lymphocyte Stimulatory Antigens, pubmed-meshheading:2150922-Organ Culture Techniques, pubmed-meshheading:2150922-Receptors, Antigen, T-Cell, pubmed-meshheading:2150922-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:2150922-T-Lymphocytes, pubmed-meshheading:2150922-Thymus Gland
pubmed:year
1990
pubmed:articleTitle
Ligand thresholds at different stages of T cell development.
pubmed:affiliation
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510.
pubmed:publicationType
Journal Article, Comparative Study