Linked Life Data

21508487

Source:http://linkedlifedata.com/resource/pubmed/id/21508487

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2011-4-21
pubmed:abstractText
A novel technique for analyzing the conformational fluctuations of a single, surface-tethered DNA molecule by fluorescence microscopy is reported. Attaching a nanometer-sized fluorescent quantum dot to the free end of a ?-phage DNA molecule allows us to study the fluctuations of a native DNA molecule without the mechanical properties being altered by fluorescent dye staining. We report on the investigation of single-tethered DNA in both the unperturbed and the shear flow induced stretched state. The dependence of the observed fractional extension and the magnitude of fluctuations on the shear rate can be qualitatively interpreted by Brochard's stem-and-flower model. The cyclic dynamics of a DNA molecule is directly observed in the shear flow experiment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1361-648X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
11
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
184119
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Analysis of the fluctuations of a single-tethered, quantum-dot labeled DNA molecule in shear flow.
pubmed:affiliation
Professur für Physikalische Chemie, Mess- und Sensortechnik, Technische Universität Dresden, 01062 Dresden, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't