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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2011-4-21
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pubmed:abstractText |
The antitumor activity of histone deacetylase inhibitors (HDACI) on multidrug-resistant sarcoma cell lines has not been previously described. Treatment of multidrug-resistant sarcoma cell lines with HDACI PCI-24781 resulted in dose-dependent accumulation of acetylated histone, p21 and poly(ADP-ribose)polymerase (PARP) cleavage products. Growth of these cell lines was inhibited by PCI-24781 at IC(50) of 0.43 to 2.7. When we looked for synergy of PCI-24781 with chemotherapeutic agents, we found that PCI-24781 reverses drug resistance in all four multidrug-resistant sarcoma cell lines and synergizes with chemotherapeutic agents to enhance caspase-3/-7 activity. Expression of RAD51 (a marker for DNA double-strand break repair) was inhibited and the expression of GADD45? (a marker for growth arrest and DNA-damage) was induced by PCI-24781 in multidrug-resistant sarcoma cell lines. In conclusion, HDACI PCI-24781 synergizes with chemotherapeutic drugs to induce apoptosis and reverses drug resistance in multidrug-resistant sarcoma cell lines.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/CRA 024781,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GADD45A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxamic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/P-Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/P-glycoprotein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1791-7530
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1115-23
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pubmed:meshHeading |
pubmed-meshheading:21508354-Antineoplastic Agents,
pubmed-meshheading:21508354-Apoptosis,
pubmed-meshheading:21508354-Benzofurans,
pubmed-meshheading:21508354-Blotting, Western,
pubmed-meshheading:21508354-Caspases,
pubmed-meshheading:21508354-Cell Cycle Proteins,
pubmed-meshheading:21508354-Cell Line, Tumor,
pubmed-meshheading:21508354-Cell Proliferation,
pubmed-meshheading:21508354-Drug Resistance, Multiple,
pubmed-meshheading:21508354-Drug Resistance, Neoplasm,
pubmed-meshheading:21508354-Histone Deacetylase Inhibitors,
pubmed-meshheading:21508354-Humans,
pubmed-meshheading:21508354-Hydroxamic Acids,
pubmed-meshheading:21508354-Nuclear Proteins,
pubmed-meshheading:21508354-P-Glycoproteins,
pubmed-meshheading:21508354-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:21508354-Sarcoma
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pubmed:year |
2011
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pubmed:articleTitle |
Histone deacetylase inhibitor PCI-24781 enhances chemotherapy-induced apoptosis in multidrug-resistant sarcoma cell lines.
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pubmed:affiliation |
Department of Orthopaedic Surgery, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China. yangcaom@gmail.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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