Source:http://linkedlifedata.com/resource/pubmed/id/21508165
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2011-6-2
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| pubmed:abstractText |
To construct a universal vaccine against mastitis induced by either Streptococcus agalactiae or Staphylococcus aureus, the B cell epitopes of the surface immunogenic protein (Sip) from S. agalactiae and clumping factor A (ClfA) from S. aureus were analyzed and predicted. sip-clfA, a novel chimeric B cell epitope-based gene, was obtained by overlap PCR, and then the recombinant Sip-ClfA (rSip-ClfA) was expressed and purified. rSip-ClfA and inactivated S. agalactiae and S. aureus were formulated into different vaccines with mineral oil as the adjuvant and evaluated in mouse models. The rSip-ClfA vaccination induced immunoglobulin G (IgG) titers higher than those seen in groups immunized with inactivated bacteria. Furthermore, the response to rSip-ClfA immunization was characterized as having a dominant IgG1 subtype, whereas both bacterial immunizations produced similar levels of IgG1 and IgG2a. The antiserum capacities for opsonizing adhesion and phagocytosis were significantly greater in the rSip-ClfA immunization group than in the killed-bacterium immunization groups (P < 0.05). The immunized lactating mice were challenged with either S. agalactiae or S. aureus via the intramammary route. At 24 h postinfection, the numbers of bacteria recovered from the mammary glands in the rSip-ClfA group were >5-fold lower than those in both inactivated-bacterium groups (P < 0.01). Histopathological examination of the mammary glands showed that rSip-ClfA immunization provided better protection of mammary gland tissue integrity against both S. agalactiae and S. aureus challenges. Thus, the recombinant protein rSip-ClfA would be a promising vaccine candidate against mastitis induced by either S. agalactiae or S. aureus.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ClfA protein, Staphylococcus aureus,
http://linkedlifedata.com/resource/pubmed/chemical/Coagulase,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, B-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Mineral Oil,
http://linkedlifedata.com/resource/pubmed/chemical/Opsonin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Staphylococcal Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
|
| pubmed:issn |
1556-679X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
18
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
893-900
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| pubmed:meshHeading |
pubmed-meshheading:21508165-Adjuvants, Immunologic,
pubmed-meshheading:21508165-Animals,
pubmed-meshheading:21508165-Antibodies, Bacterial,
pubmed-meshheading:21508165-Bacterial Load,
pubmed-meshheading:21508165-Bacterial Proteins,
pubmed-meshheading:21508165-Cattle,
pubmed-meshheading:21508165-Coagulase,
pubmed-meshheading:21508165-Epitopes, B-Lymphocyte,
pubmed-meshheading:21508165-Female,
pubmed-meshheading:21508165-Histocytochemistry,
pubmed-meshheading:21508165-Immunoglobulin G,
pubmed-meshheading:21508165-Mammary Glands, Animal,
pubmed-meshheading:21508165-Mastitis,
pubmed-meshheading:21508165-Mice,
pubmed-meshheading:21508165-Mice, Inbred BALB C,
pubmed-meshheading:21508165-Mineral Oil,
pubmed-meshheading:21508165-Opsonin Proteins,
pubmed-meshheading:21508165-Phagocytosis,
pubmed-meshheading:21508165-Recombinant Proteins,
pubmed-meshheading:21508165-Staphylococcal Vaccines,
pubmed-meshheading:21508165-Staphylococcus aureus,
pubmed-meshheading:21508165-Streptococcus agalactiae,
pubmed-meshheading:21508165-Vaccines, Synthetic
|
| pubmed:year |
2011
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| pubmed:articleTitle |
Evaluation of a novel chimeric B cell epitope-based vaccine against mastitis induced by either Streptococcus agalactiae or Staphylococcus aureus in mice.
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| pubmed:affiliation |
The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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