Linked Life Data

21505270

Source:http://linkedlifedata.com/resource/pubmed/id/21505270

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-4-20
pubmed:abstractText
Acute-on-chronic hepatitis B liver failure (ACHBLF) refers to liver failure occurring in patients with chronic hepatitis B (CHB) related liver diseases. Interferon-? (IFN-?) plays an important role in the exacerbation of liver function. However, the exact mechanism, by which IFN-? mediates ACHBLF, is not fully understood. Forty patients with ACHBLF, fifteen patients with CHB and ten healthy controls were included in this present study. ELISA was performed to measure the level of serum IFN-?. The methylation status of IFN-? promoter in peripheral blood mononuclear cells (PBMCs) was determined using methylation-specific PCR. Model for End-stage Liver Disease (MELD) scoring was performed for evaluating the severity of liver failure. The serum level of IFN-? in patients with ACHBLF or CHB was significantly lower than that in healthy controls, while the serum IFN-? level in ACHBLF patients was significantly higher than that in CHB patients. In ACHBLF patients, the level of IFN-? was positively correlated with total bilirubin and MELD score, but negatively correlated with prothrombin time activity. These results suggest the involvement of IFN-? in the pathogenesis of ACHBLF. Importantly, the degree of methylation of the IFN-? gene promoter in ACHBLF patients (60%, 24/40) was significantly lower than that in CHB patients (93%, 14/15), but was higher than that in the control group (20%, 2/10). Furthermore, in ACHBLF patients, the serum IFN-? level was significantly higher in unmethylation group than that in methylation group. In conclusion, enhanced demethylation of IFN-? gene promoter in PBMCs may be associated with the onset of ACHBLF.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1349-3329
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
224
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-9
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Enhanced demethylation of interferon-? gene promoter in peripheral blood mononuclear cells is associated with acute-on-chronic hepatitis B liver failure.
pubmed:affiliation
Department of Hepatology, Qilu Hospital of Shandong University, Jinan, PR China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't