Linked Life Data

21505051

Source:http://linkedlifedata.com/resource/pubmed/id/21505051

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-6-23
pubmed:abstractText
This study tested the hypotheses that prostaglandin (PG) F(2?) increases expression of genes related to recruitment of leukocytes in mature but not early corpus luteum (CL) and that insensitivity to PGF(2?) action in early CL is dependent on high intraluteal progesterone (P4) concentrations. Experiment 1 examined early (0.5 h) and late (10 h) in vivo effects of PGF(2?) on mature (d 17 of pseudopregnancy) and early (d 9) porcine CL. Real-time PCR was used to measure mRNA for chemokines (IL8, CXCL2, CCL2, CCL8, CCL4, CCL11) and chemokine receptors (CCR1, CCR2, CXCR2, CCR5). Western blotting was used to measure protein expression and phosphorylation of nuclear factor-?B proteins. Treatment with PGF(2?) for 10 h increased mRNA for almost all of these genes (all expect CXCL2 and CCL11) in d 17 CL but not d 9 CL. Treatment with PGF(2?) also led to greater phosphorylation of nuclear factor-?B-1A protein in d 17 than d 9 CL. Experiment 2 had a 2 × 2 factorial design with d 9 gilts treated or not treated with epostane (3?-hydroxysteroid dehydrogenase inhibitor to suppress intraluteal P4) and treated or not treated with PGF(2?). Treatment with PGF(2?) (10 h) or epostane alone did not induce expression of any of these genes in d 9 CL. However, PGF(2?) + epostane increased expression of all of these genes except CCL11. In conclusion, PGF(2?) increases mRNA for chemokines and chemokine receptors in mature CL with similar PGF(2?) effects induced in early CL if intraluteal P4 is suppressed prior to PGF(2?) treatment.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1945-7170
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2797-805
pubmed:meshHeading
pubmed-meshheading:21505051-3-Hydroxysteroid Dehydrogenases, pubmed-meshheading:21505051-Androstenols, pubmed-meshheading:21505051-Animals, pubmed-meshheading:21505051-Chemokines, pubmed-meshheading:21505051-Corpus Luteum, pubmed-meshheading:21505051-Dinoprost, pubmed-meshheading:21505051-Enzyme Inhibitors, pubmed-meshheading:21505051-Female, pubmed-meshheading:21505051-Luteinization, pubmed-meshheading:21505051-NF-kappa B, pubmed-meshheading:21505051-Phosphorylation, pubmed-meshheading:21505051-Progesterone, pubmed-meshheading:21505051-Protein Processing, Post-Translational, pubmed-meshheading:21505051-Pseudopregnancy, pubmed-meshheading:21505051-RNA, Messenger, pubmed-meshheading:21505051-Random Allocation, pubmed-meshheading:21505051-Receptors, Chemokine, pubmed-meshheading:21505051-Signal Transduction, pubmed-meshheading:21505051-Sus scrofa, pubmed-meshheading:21505051-Up-Regulation
pubmed:year
2011
pubmed:articleTitle
Induction of mRNA for chemokines and chemokine receptors by prostaglandin F2? is dependent upon stage of the porcine corpus luteum and intraluteal progesterone.
pubmed:affiliation
Endocrinology-Reproductive Physiology Program and Department of Dairy Science, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural