Source:http://linkedlifedata.com/resource/pubmed/id/21504904
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
24
|
| pubmed:dateCreated |
2011-6-13
|
| pubmed:abstractText |
In plant organelles, RNA editing is a post-transcriptional mechanism that converts specific cytidines to uridines in RNA of both mitochondria and plastids, altering the information encoded by the gene. The cytidine to be edited is determined by a cis-element surrounding the editing site that is specifically recognized and bound by a trans-acting factor. All the trans-acting editing factors identified so far in plant organelles are members of a large protein family, the pentatricopeptide repeat (PPR) proteins. We have identified the Organelle Transcript Processing 87 (OTP87) gene, which is required for RNA editing of the nad7-C24 and atp1-C1178 sites in Arabidopsis mitochondria. OTP87 encodes an E-subclass PPR protein with an unusually short E-domain. The recombinant protein expressed in Escherichia coli specifically binds to RNAs comprising 30 nucleotides upstream and 10 nucleotides downstream of the nad7-C24 and atp1-C1178 editing sites. The loss-of-function of OTP87 results in small plants with growth and developmental delays. In the otp87 mutant, the amount of assembled respiratory complex V (ATP synthase) is highly reduced compared with the wild type suggesting that the amino acid alteration in ATP1 caused by loss of editing at the atp1-C1178 site affects complex V assembly in mitochondria.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorophyll,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proton-Translocating...,
http://linkedlifedata.com/resource/pubmed/chemical/NADH Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proton-Translocating ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1083-351X
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
17
|
| pubmed:volume |
286
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
21361-71
|
| pubmed:meshHeading |
pubmed-meshheading:21504904-Arabidopsis,
pubmed-meshheading:21504904-Arabidopsis Proteins,
pubmed-meshheading:21504904-Binding Sites,
pubmed-meshheading:21504904-Chlorophyll,
pubmed-meshheading:21504904-Escherichia coli,
pubmed-meshheading:21504904-Gene Expression Regulation, Plant,
pubmed-meshheading:21504904-Genetic Complementation Test,
pubmed-meshheading:21504904-Mitochondria,
pubmed-meshheading:21504904-Mitochondrial Proton-Translocating ATPases,
pubmed-meshheading:21504904-Models, Genetic,
pubmed-meshheading:21504904-Mutation,
pubmed-meshheading:21504904-NADH Dehydrogenase,
pubmed-meshheading:21504904-Peptides,
pubmed-meshheading:21504904-Phenotype,
pubmed-meshheading:21504904-Plant Proteins,
pubmed-meshheading:21504904-Protein Structure, Tertiary,
pubmed-meshheading:21504904-Proton-Translocating ATPases,
pubmed-meshheading:21504904-RNA Editing,
pubmed-meshheading:21504904-RNA-Binding Proteins
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
The pentatricopeptide repeat protein OTP87 is essential for RNA editing of nad7 and atp1 transcripts in Arabidopsis mitochondria.
|
| pubmed:affiliation |
Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley 6009 Western Australia, Australia. khammani@uoregon.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|