Source:http://linkedlifedata.com/resource/pubmed/id/21497715
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2011-4-18
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| pubmed:abstractText |
Dynamin family proteins in eukaryotic cells assemble into rings or spirals on the surface of membranes and pinch the membranes. We found 21 dynamin-related protein (DRP) genes in the Physcomitrella patens genome. Phylogenetic analysis indicated that three of them (PpDRP5B-1, PpDRP5B-2, and PpDRP5B-3) showed robust monophyly with Arabidopsis thaliana DRP5B and Cyanidioschyzon merolae CmDnm2, both of which are related to plastid division. Quantitative RT-PCR analysis showed that the amounts of DRP5B-3 transcripts were 14-fold and 8-fold higher than those of DRP5B-1 and DRP5B-2, respectively. We generated PpDRP5B knockout transformants for each of these genes. Subapical protonemata cells in wild-type plants had an average of 47 chloroplasts. The cells in the PpDRP5B-3 knockout transformant had slightly enlarged chloroplasts, with an average chloroplast number of 28, whereas the PpDRP5B-1 and 5B-2 knockout lines had no effect on chloroplast number in P. patens. To analyze function of each PpDRP5B gene, we generated double- and triple-knockout lines. Whereas there were 32 chloroplasts in a cell of the PpDRP5B-1/5B-2 double-knockout lines, the triple-knockout line had only a few macrochloroplasts. A transient expression assay with the triple-knockout line demonstrated that the PpDRP5B-3 gene could recover the normal chloroplast phenotype.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
1873-2259
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
180
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
789-95
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| pubmed:meshHeading |
pubmed-meshheading:21497715-Bryopsida,
pubmed-meshheading:21497715-Chloroplasts,
pubmed-meshheading:21497715-Dynamins,
pubmed-meshheading:21497715-Gene Expression Regulation, Plant,
pubmed-meshheading:21497715-Gene Knockout Techniques,
pubmed-meshheading:21497715-Genes, Plant,
pubmed-meshheading:21497715-Genetic Complementation Test,
pubmed-meshheading:21497715-Genome, Plant,
pubmed-meshheading:21497715-Green Fluorescent Proteins,
pubmed-meshheading:21497715-Microscopy, Electron,
pubmed-meshheading:21497715-Microscopy, Fluorescence,
pubmed-meshheading:21497715-Phenotype,
pubmed-meshheading:21497715-Phylogeny,
pubmed-meshheading:21497715-Plastids,
pubmed-meshheading:21497715-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21497715-Sequence Deletion,
pubmed-meshheading:21497715-Transformation, Genetic
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| pubmed:year |
2011
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| pubmed:articleTitle |
Three dynamin-related protein 5B genes are related to plastid division in Physcomitrella patens.
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| pubmed:affiliation |
Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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