Linked Life Data

21488617

Source:http://linkedlifedata.com/resource/pubmed/id/21488617

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pubmed-article:21488617pubmed:dateCreated2011-5-10lld:pubmed
pubmed-article:21488617pubmed:abstractTextA biocatalyst was synthesized by immobilizing human carbonic anhydrase onto gold nanoparticles assembled over amine/thiol-functionalized mesoporous SBA-15. The physicochemical properties of the functionalized mesoporous SBA-15 were obtained by XRD, BET, FE SEM, HR TEM, EDS, and zeta potential analysis. The biocatalytic performance was studied for para-nitrophenyl acetate (p-NPA) hydrolysis. The kinetic parameters K(m) were found to be 22.35 and 27.75 mM, and K(cat)/K(m) values were 1514.09 and 1612.25 M(-1) s(-1) for HCA immobilized on gold nanoparticles assembled on amine/thiol-functionalized mesoporous SBA-15 (HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15), respectively. These HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15 were investigated for biocatalytic hydration of CO(2) and its precipitation as CaCO(3). The amount of CaCO(3) precipitated over HCA/Au/MPTES/SBA-15 was nearly the same as that precipitated over free HCA. Storage stability and reusability studies suggested that HCA/Au/MPTES/SBA-15 retained its activity even after 20 days storage at 25 °C and 20 recycling runs. The present results demonstrate that HCA/Au/MPTES/SBA-15 and HCA/Au/APTES/SBA-15 are highly efficient potential nanobiocatalysts for industrial-scale CO(2) sequestration.lld:pubmed
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pubmed-article:21488617pubmed:authorpubmed-author:AlagarMuthuka...lld:pubmed
pubmed-article:21488617pubmed:authorpubmed-author:LeeSi HyunSHlld:pubmed
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pubmed-article:21488617pubmed:authorpubmed-author:JeongSoon...lld:pubmed
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pubmed-article:21488617pubmed:year2011lld:pubmed
pubmed-article:21488617pubmed:articleTitleImmobilization of human carbonic anhydrase on gold nanoparticles assembled onto amine/thiol-functionalized mesoporous SBA-15 for biomimetic sequestration of CO2.lld:pubmed
pubmed-article:21488617pubmed:affiliationKorea Institute of Energy Research, Daejeon 305-343, Korea.lld:pubmed
pubmed-article:21488617pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21488617pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed