Source:http://linkedlifedata.com/resource/pubmed/id/21488617
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2011-5-10
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| pubmed:abstractText |
A biocatalyst was synthesized by immobilizing human carbonic anhydrase onto gold nanoparticles assembled over amine/thiol-functionalized mesoporous SBA-15. The physicochemical properties of the functionalized mesoporous SBA-15 were obtained by XRD, BET, FE SEM, HR TEM, EDS, and zeta potential analysis. The biocatalytic performance was studied for para-nitrophenyl acetate (p-NPA) hydrolysis. The kinetic parameters K(m) were found to be 22.35 and 27.75 mM, and K(cat)/K(m) values were 1514.09 and 1612.25 M(-1) s(-1) for HCA immobilized on gold nanoparticles assembled on amine/thiol-functionalized mesoporous SBA-15 (HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15), respectively. These HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15 were investigated for biocatalytic hydration of CO(2) and its precipitation as CaCO(3). The amount of CaCO(3) precipitated over HCA/Au/MPTES/SBA-15 was nearly the same as that precipitated over free HCA. Storage stability and reusability studies suggested that HCA/Au/MPTES/SBA-15 retained its activity even after 20 days storage at 25 °C and 20 recycling runs. The present results demonstrate that HCA/Au/MPTES/SBA-15 and HCA/Au/APTES/SBA-15 are highly efficient potential nanobiocatalysts for industrial-scale CO(2) sequestration.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amines,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonic Anhydrases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzymes, Immobilized,
http://linkedlifedata.com/resource/pubmed/chemical/Gold,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylacetates,
http://linkedlifedata.com/resource/pubmed/chemical/SBA-15,
http://linkedlifedata.com/resource/pubmed/chemical/Silanes,
http://linkedlifedata.com/resource/pubmed/chemical/Silicon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfhydryl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/amino-propyl-triethoxysilane,
http://linkedlifedata.com/resource/pubmed/chemical/phenylacetic acid
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| pubmed:status |
MEDLINE
|
| pubmed:month |
May
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| pubmed:issn |
1520-5827
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
17
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| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6227-34
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| pubmed:meshHeading |
pubmed-meshheading:21488617-Amines,
pubmed-meshheading:21488617-Biocatalysis,
pubmed-meshheading:21488617-Biomimetics,
pubmed-meshheading:21488617-Carbon Dioxide,
pubmed-meshheading:21488617-Carbonic Anhydrases,
pubmed-meshheading:21488617-Enzymes, Immobilized,
pubmed-meshheading:21488617-Gold,
pubmed-meshheading:21488617-Humans,
pubmed-meshheading:21488617-Hydrolysis,
pubmed-meshheading:21488617-Metal Nanoparticles,
pubmed-meshheading:21488617-Phenylacetates,
pubmed-meshheading:21488617-Porosity,
pubmed-meshheading:21488617-Silanes,
pubmed-meshheading:21488617-Silicon Dioxide,
pubmed-meshheading:21488617-Sulfhydryl Compounds
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
Immobilization of human carbonic anhydrase on gold nanoparticles assembled onto amine/thiol-functionalized mesoporous SBA-15 for biomimetic sequestration of CO2.
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| pubmed:affiliation |
Korea Institute of Energy Research, Daejeon 305-343, Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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