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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-5-30
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pubmed:abstractText |
Vascular endothelial growth factor C (VEGF-C) is a key regulator of angiogenesis and lymphangiogenesis. VEGF-C is also implicated in the development of esophageal cancer. We investigated the mRNA levels of VEGF-C and its receptors in 38 esophageal squamous cell carcinoma specimens (ESCCs) and matched adjacent normal esophageal tissues via real-time PCR. The mRNA levels of VEGF-C, VEGFR-2 and VEGFR-3 were significantly upregulated in ESCCs versus respective side normal tissues. To explore the influence of VEGF-C on esophageal cancer progression, the expression of VEGF-C was manipulated in esophageal cancer cell lines TE-1 and Eca-109. VEGF-C transcription, translation and secretion were significantly enhanced in cells stably transfected with a VEGF-C overexpression vector or attenuated in VEGF-C shRNA-transfected cell lines. In vitro, TE-1 cells stably transfected with a VEGF-C overexpression vector exhibited an increased rate of cell proliferation, migration and focus formation, whereas knockdown of VEGF-C inhibited cell proliferation, migration and focus formation. Similar results were obtained for Eca-109 cells. VEGF-C mediated biological function through transcription of CNTN-1, which is implicated in tumor invasion and metastasis. The expression of VEGF-C was correlated with that of CNTN-1 and cell proliferation and migration induced by VEGF-C were reversed by silencing of CNTN-1. In addition, nude mice inoculated with VEGF-C shRNA-transfected cells exhibited a significantly decreased tumor size in vivo via reduced VEGFR-2 and VEGFR-3 phosphorylation and microvessel formation. VEGF-C upregulation may be involved in esophageal tumor progression. Vector-based RNA interference (RNAi) targeting VEGF-C is a potential therapeutic method for human esophageal carcinoma.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1096-0023
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pubmed:author |
pubmed-author:LiuBingtuanB,
pubmed-author:LiuPengfeiP,
pubmed-author:ShenWeidongW,
pubmed-author:ShiRuihuaR,
pubmed-author:WangFangjunF,
pubmed-author:WuYeY,
pubmed-author:XiangBinB,
pubmed-author:XieLingL,
pubmed-author:ZhangShuyuS,
pubmed-author:ZhouJundongJ,
pubmed-author:ZhuHongH,
pubmed-author:ZhuXiaozhongX
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8-17
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pubmed:meshHeading |
pubmed-meshheading:21482472-Aged,
pubmed-meshheading:21482472-Animals,
pubmed-meshheading:21482472-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:21482472-Cell Line, Tumor,
pubmed-meshheading:21482472-Cell Movement,
pubmed-meshheading:21482472-Cell Proliferation,
pubmed-meshheading:21482472-Contactin 1,
pubmed-meshheading:21482472-Down-Regulation,
pubmed-meshheading:21482472-Drug Resistance, Neoplasm,
pubmed-meshheading:21482472-Esophageal Neoplasms,
pubmed-meshheading:21482472-Female,
pubmed-meshheading:21482472-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:21482472-Gene Knockdown Techniques,
pubmed-meshheading:21482472-Gene Silencing,
pubmed-meshheading:21482472-Humans,
pubmed-meshheading:21482472-Male,
pubmed-meshheading:21482472-Mice,
pubmed-meshheading:21482472-Microvessels,
pubmed-meshheading:21482472-Middle Aged,
pubmed-meshheading:21482472-Precancerous Conditions,
pubmed-meshheading:21482472-Promoter Regions, Genetic,
pubmed-meshheading:21482472-Protein Binding,
pubmed-meshheading:21482472-Vascular Endothelial Growth Factor C,
pubmed-meshheading:21482472-Vascular Endothelial Growth Factor Receptor-2,
pubmed-meshheading:21482472-Vascular Endothelial Growth Factor Receptor-3,
pubmed-meshheading:21482472-Xenograft Model Antitumor Assays
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pubmed:year |
2011
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pubmed:articleTitle |
VEGF-C promotes the development of esophageal cancer via regulating CNTN-1 expression.
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pubmed:affiliation |
Department of Gastroenterology, The Affiliated Jiangyin Hospital of Southeast University, Jiangyin 214400, China. jylpf@163.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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