Linked Life Data

21482286

Source:http://linkedlifedata.com/resource/pubmed/id/21482286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-4-12
pubmed:abstractText
In this study, a frozen water phase method for logD measurement using a 96-well plate was developed. In the case of logD measurement of compounds, the problem of octanol contamination often occurs; in lipophilic compounds, the concentration of the octanol phase is much higher than that of the water phase. When the water phase is separated from the octanol phase, a small amount of octanol phase contamination could strongly influence the concentration of the water phase. To avoid this problem, the frozen water phase method was developed. The water phase was frozen in liquid nitrogen and then the unfrozen octanol phase was removed. To remove the portion of the octanol remaining on the frozen water phase, the surface of the frozen water phase was washed with octanol and water/ethanol (50/50, v/v). The validity of the method was confirmed by results of commercially available drugs at the logD range from 0 to 4. Further, it was found that this method had the ability to evaluate the pH-logD profile of compounds in the range from pH 2 to pH 12. As a result, we developed the convenient and accurate method that is effective in preventing contamination with a wide dynamic range.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-3573
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
809-13
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Frozen water phase method for logD measurement using a 96-well plate.
pubmed:affiliation
Analytical Research, CMC Japan, Pharmaceutical Science & Technology Function Unit, Eisai Co. Ltd., 1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. t5-yamashita@hhc.eisai.co.jp
pubmed:publicationType
Journal Article, Validation Studies