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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-1-24
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pubmed:abstractText |
Stimulation of Jurkat T cells with antibodies against the T cell receptor/CD3 complex induces a rise in the intracellular concentration of Ca2+ within seconds. The inositol phosphate-dependent Ca2+ mobilization induced by OKT3 was completely abrogated when Jurkat cells were pretreated for 1 min with the phorbol 12-myristate 13-acetate TPA (10nM), a concentration which activates protein kinase C (PKC). The effects of TPA on the Ca2+ fluxes were insensitive to treatment of the cells with known PKC inhibitors (H-7 and staurosporin) under conditions where the PKC-mediated phosphorylation was blocked. Furthermore, another activator of PKC, mezerein, inhibited the Ca2+ signal induced by OKT3. This inhibition, however, could completely be reversed by pretreatment with H-7 or staurosporine. We conclude that the TPA-mediated inhibition of Ca2+ fluxes in Jurkat T cells largely acts through a PKC-independent pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
396-400
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2147852-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine,
pubmed-meshheading:2147852-Antibodies, Monoclonal,
pubmed-meshheading:2147852-Antigens, CD,
pubmed-meshheading:2147852-Antigens, CD3,
pubmed-meshheading:2147852-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2147852-Calcium,
pubmed-meshheading:2147852-Cell Line,
pubmed-meshheading:2147852-Fura-2,
pubmed-meshheading:2147852-Humans,
pubmed-meshheading:2147852-Inositol,
pubmed-meshheading:2147852-Inositol Phosphates,
pubmed-meshheading:2147852-Isoquinolines,
pubmed-meshheading:2147852-Phosphorylation,
pubmed-meshheading:2147852-Piperazines,
pubmed-meshheading:2147852-Protein Kinase C,
pubmed-meshheading:2147852-Receptors, Antigen, T-Cell,
pubmed-meshheading:2147852-Spectrometry, Fluorescence,
pubmed-meshheading:2147852-T-Lymphocytes,
pubmed-meshheading:2147852-Tetradecanoylphorbol Acetate
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pubmed:year |
1990
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pubmed:articleTitle |
Phorbol 12-myristate 13-acetate (TPA) blocks CD3-mediated Ca2+ mobilization in Jurkat T cells independently of protein kinase C activation.
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pubmed:affiliation |
Department of Pathology, University of Helsinki, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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