| pubmed-article:21474333 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0221908 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0031715 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0754728 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C1334474 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C1366765 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C1440080 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C0038891 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:21474333 | lifeskim:mentions | umls-concept:C1705294 | lld:lifeskim |
| pubmed-article:21474333 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:21474333 | pubmed:dateCreated | 2011-5-30 | lld:pubmed |
| pubmed-article:21474333 | pubmed:abstractText | Surfactant proteins (SPs) and toll-like receptors (TLRs) contribute to regulation of sepsis-induced acute lung injury. Lipopolysaccharide (LPS) is one of the major causes of septic shock. This study was designed to evaluate the effects of LPS on the regulation of tlr-2 and sp-a gene expression in human alveolar epithelial A549 cells and the possible mechanisms. Exposure of A549 cells to LPS increased the expressions of TLR2 and SP-A mRNA and protein in time-dependent manners. A search using a bioinformatic approach found that there are several nuclear factor kappa-B (NF-?B)-DNA-binding motifs in the promoter region of the tlr2 and sp-a genes. Immunoblotting analyses revealed that exposure to LPS time-dependently enhanced the translocation of NF-?B from the cytoplasm to nuclei. Analyses of an electrophoretic mobility shift assay further showed that LPS augmented the transactivation activity of NF-?B to its consensus oligonucleotides in A549cells. Sequentially, treatment of A549 cells with LPS increased phosphorylation of extracellular signal-regulated kinase (ERK)1/2, p38-mitogen-activated protein kinase (p38MAPK), and MAPK kinase-1 (MEK1). Pretreatment with PD98059, an inhibitor of ERK1/2, significantly decreased LPS-induced TLR2 and SP-A mRNA expression. | lld:pubmed |
| pubmed-article:21474333 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21474333 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21474333 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21474333 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:21474333 | pubmed:issn | 1096-0023 | lld:pubmed |
| pubmed-article:21474333 | pubmed:author | pubmed-author:ChenTa-LiangT... | lld:pubmed |
| pubmed-article:21474333 | pubmed:author | pubmed-author:ChenRuei-Ming... | lld:pubmed |
| pubmed-article:21474333 | pubmed:author | pubmed-author:WuTsu-TuanTT | lld:pubmed |
| pubmed-article:21474333 | pubmed:author | pubmed-author:TaiYu-TingYT | lld:pubmed |
| pubmed-article:21474333 | pubmed:author | pubmed-author:CherngYih-Giu... | lld:pubmed |
| pubmed-article:21474333 | pubmed:author | pubmed-author:LoonWun-SingW... | lld:pubmed |
| pubmed-article:21474333 | pubmed:copyrightInfo | Copyright © 2011 Elsevier Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:21474333 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21474333 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:21474333 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21474333 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21474333 | pubmed:pagination | 40-7 | lld:pubmed |
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| pubmed-article:21474333 | pubmed:meshHeading | pubmed-meshheading:21474333... | lld:pubmed |
| pubmed-article:21474333 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21474333 | pubmed:articleTitle | Lipopolysaccharide stimulates syntheses of toll-like receptor 2 and surfactant protein-A in human alveolar epithelial A549 cells through upregulating phosphorylation of MEK1 and ERK1/2 and sequential activation of NF-?B. | lld:pubmed |
| pubmed-article:21474333 | pubmed:affiliation | Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan. | lld:pubmed |
| pubmed-article:21474333 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21474333 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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