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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0023810,
umls-concept:C0031715,
umls-concept:C0038891,
umls-concept:C0086418,
umls-concept:C0220781,
umls-concept:C0221908,
umls-concept:C0754728,
umls-concept:C1334474,
umls-concept:C1366765,
umls-concept:C1440080,
umls-concept:C1705294,
umls-concept:C1879547,
umls-concept:C1883254
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pubmed:issue |
1
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pubmed:dateCreated |
2011-5-30
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pubmed:abstractText |
Surfactant proteins (SPs) and toll-like receptors (TLRs) contribute to regulation of sepsis-induced acute lung injury. Lipopolysaccharide (LPS) is one of the major causes of septic shock. This study was designed to evaluate the effects of LPS on the regulation of tlr-2 and sp-a gene expression in human alveolar epithelial A549 cells and the possible mechanisms. Exposure of A549 cells to LPS increased the expressions of TLR2 and SP-A mRNA and protein in time-dependent manners. A search using a bioinformatic approach found that there are several nuclear factor kappa-B (NF-?B)-DNA-binding motifs in the promoter region of the tlr2 and sp-a genes. Immunoblotting analyses revealed that exposure to LPS time-dependently enhanced the translocation of NF-?B from the cytoplasm to nuclei. Analyses of an electrophoretic mobility shift assay further showed that LPS augmented the transactivation activity of NF-?B to its consensus oligonucleotides in A549cells. Sequentially, treatment of A549 cells with LPS increased phosphorylation of extracellular signal-regulated kinase (ERK)1/2, p38-mitogen-activated protein kinase (p38MAPK), and MAPK kinase-1 (MEK1). Pretreatment with PD98059, an inhibitor of ERK1/2, significantly decreased LPS-induced TLR2 and SP-A mRNA expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/MAP2K1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SFTPA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TLR2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 2,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1096-0023
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
40-7
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pubmed:meshHeading |
pubmed-meshheading:21474333-Cell Line, Tumor,
pubmed-meshheading:21474333-Cell Shape,
pubmed-meshheading:21474333-Cell Survival,
pubmed-meshheading:21474333-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:21474333-Flavonoids,
pubmed-meshheading:21474333-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:21474333-Humans,
pubmed-meshheading:21474333-Lipopolysaccharides,
pubmed-meshheading:21474333-MAP Kinase Kinase 1,
pubmed-meshheading:21474333-Models, Biological,
pubmed-meshheading:21474333-NF-kappa B,
pubmed-meshheading:21474333-Phosphorylation,
pubmed-meshheading:21474333-Pneumocytes,
pubmed-meshheading:21474333-Protein Transport,
pubmed-meshheading:21474333-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:21474333-RNA, Messenger,
pubmed-meshheading:21474333-Time Factors,
pubmed-meshheading:21474333-Toll-Like Receptor 2,
pubmed-meshheading:21474333-Transcriptional Activation,
pubmed-meshheading:21474333-Up-Regulation,
pubmed-meshheading:21474333-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2011
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pubmed:articleTitle |
Lipopolysaccharide stimulates syntheses of toll-like receptor 2 and surfactant protein-A in human alveolar epithelial A549 cells through upregulating phosphorylation of MEK1 and ERK1/2 and sequential activation of NF-?B.
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pubmed:affiliation |
Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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