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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2011-4-20
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pubmed:abstractText |
Approximately half of the mitochondrial genome inherent within 546 individual Atlantic salmon (Salmo salar) derived from across the species' North Atlantic range, was selectively amplified with a novel combination of standard PCR and pyro-sequencing in a single run using 454 Titanium FLX technology (Roche, 454 Life Sciences). A unique combination of barcoded primers and a partitioned sequencing plate was employed to designate each sequence read to its original sample. The sequence reads were aligned according to the S. salar mitochondrial reference sequence (NC_001960.1), with the objective of identifying single nucleotide polymorphisms (SNPs). They were validated if they met with the following three stringent criteria: (i) sequence reads were produced from both DNA strands; (ii) SNPs were confirmed in a minimum of 90% of replicate sequence reads; and (iii) SNPs occurred in more than one individual.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-10395891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-11251790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-12296947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-12466990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-14631358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-16056220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-16355404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-18297082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-18660809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-18826589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-18842124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-18854868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19023400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19055830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19133128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19386042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19604363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19647712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19775472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-19939274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21473771-20483203
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2164
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pubmed:author |
pubmed-author:BjornsdottirSnaedisS,
pubmed-author:ConsuegraSoniaS,
pubmed-author:FridjonssonOlafurO,
pubmed-author:HjorleifsdottirSigridurS,
pubmed-author:KnoxDavidD,
pubmed-author:MagnusdottirSteinunnS,
pubmed-author:OlafsdottirGudbjorgG,
pubmed-author:OlafssonKristinnK,
pubmed-author:TompsettScottS,
pubmed-author:VerspoorEricE,
pubmed-author:de LeanizCarlos GarciaCG
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pubmed:issnType |
Electronic
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179
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pubmed:meshHeading |
pubmed-meshheading:21473771-Animals,
pubmed-meshheading:21473771-Chromosome Mapping,
pubmed-meshheading:21473771-DNA, Mitochondrial,
pubmed-meshheading:21473771-Gene Library,
pubmed-meshheading:21473771-Genome, Mitochondrial,
pubmed-meshheading:21473771-High-Throughput Nucleotide Sequencing,
pubmed-meshheading:21473771-Polymorphism, Single Nucleotide,
pubmed-meshheading:21473771-Salmo salar,
pubmed-meshheading:21473771-Sequence Analysis, DNA
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pubmed:year |
2011
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pubmed:articleTitle |
Detection and mapping of mtDNA SNPs in Atlantic salmon using high throughput DNA sequencing.
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pubmed:affiliation |
Matís, Vínlandsleið, Reykjavík, Iceland. olafur@matis.is
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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