Linked Life Data

21472697

Source:http://linkedlifedata.com/resource/pubmed/id/21472697

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-4-7
pubmed:abstractText
The DNA ligase enzyme family catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA. This activity can seal nicks in duplex DNA or join double-stranded DNA fragments having either blunt or cohesive ends. DNA ligases are central enzymes in molecular biology, nucleic acid research, and in next-generation sequencing applications. Reaction conditions and applications for T4 DNA ligase, E. coli DNA ligase, and thermostable DNA ligases are described in this unit. These enzymes differ in their cofactor requirements, substrate specificity, and thermal stability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1934-3647
pubmed:author
pubmed:copyrightInfo
© 2011 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit3.14
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
DNA ligases.
pubmed:affiliation
New England Biolabs, Ipswich, Massachusetts, USA.
pubmed:publicationType
Journal Article