Linked Life Data

21472695

Source:http://linkedlifedata.com/resource/pubmed/id/21472695

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-4-7
pubmed:abstractText
DamIP is a new method for studying DNA-protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N(6)-adenine methylation in nearby sites in the genomic DNA. Methylated DNA fragments are enriched by immunopreciptation with an antibody that recognizes N(6)-methyladenine, and can then be used for further analysis, e.g., real-time PCR, microarray, or high-throughput sequencing. This method is simple and does not require protein-DNA crosslinking or a specific antibody to the protein of interest. This unit describes the application of this method for identification of DNA binding sites in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1934-3647
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit21.21
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
DamIP: using mutant DNA adenine methyltransferase to study DNA-protein interactions in vivo.
pubmed:affiliation
Baylor College of Medicine, Houston, Texas, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural