Linked Life Data

21471659

Source:http://linkedlifedata.com/resource/pubmed/id/21471659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
2011-4-7
pubmed:abstractText
The relationship between the higher-order structure of human metaphase chromosomes and the distribution of topoisomerase II? was analyzed by a comparison of atomic force microscope (AFM) and fluorescence microscope images of the same chromosome. AFM imaging of chromosomes in liquid revealed the presence of alternating ridges and grooves on the surfaces of the sister chromatids. In contrast, the fluorescence image of the chromosomes stained with the anti-topoisomerase II? antibody showed that the fluorescence intensity of topoisomerase II? was not uniform and that there were alternating strong and weak spots along the chromosome axes. A comparison of the AFM image with a fluorescence microscope image of the same chromosome further demonstrated that ridges and grooves corresponded to strong and weak fluorescence intensities of topoisomerase II?, respectively. These findings suggest that the distribution of topoisomerase II? has a close connection with the higher-order structure of human metaphase chromosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1349-1717
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-9
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Structural analysis of human chromosomes by atomic force and light microscopy in relation to the distribution of topoisomerase II?.
pubmed:affiliation
Division of Microscopic Anatomy and Bio-imaging, Niigata University Graduate School of Medical and Dental Science, Niigata, Japan. o-hoshi@med.niigata-u.ac.jp
pubmed:publicationType
Journal Article