Linked Life Data

21470888

Source:http://linkedlifedata.com/resource/pubmed/id/21470888

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-6-7
pubmed:abstractText
Type 2 diabetes patients exhibit a reduction in oxidative muscle fibres and an increase in glycolytic muscle fibres. In this study, we investigated whether both genetic and non-genetic factors influence the mRNA expression levels of three myosin heavy chain (MHC) genes represented in different fibre types. Specifically, we examined the MHC7 (slow-twitch oxidative fibre), MHCIIa (fast-twitch oxidative fibre) and MHCIIx/d (fast-twitch glycolytic fibre) genes in human skeletal muscle. We further investigated the use of MHC mRNA expression as a proxy to determine fibre-type composition, as measured by traditional ATP staining. Two cohorts of age-matched Swedish men were studied to determine the relationship of muscle mRNA expression of MHC7, MHCIIa, and MHCIIx/d with muscle fibre composition. A classical twin approach, including young and elderly Danish twin pairs, was utilised to examine if differences in expression levels were due to genetic or environmental factors. Although MHCIIx/d mRNA expression correlated positively with the level of type IIx/d muscle fibres in the two cohorts (P<0.05), a relatively low magnitude of correlation suggests that mRNA does not fully correlate with fibre-type composition. Heritability estimates and genetic analysis suggest that the levels of MHC7, MHCIIa and MHCIIx/d expression are primarily under non-genetic influence, and MHCIIa indicated an age-related decline. PGC-1? exhibited a positive relationship with the expression of all three MHC genes (P<0.05); meanwhile, PGC-1? related positively with MHCIIa expression and negatively with MHCIIx/d expression (P<0.05). While MHCIIa expression related positively with insulin-stimulated glucose uptake (P<0.01), MHCIIx/d expression related negatively with insulin-stimulated glucose uptake (P<0.05). Our findings suggest that the expression levels of the MHC genes are associated with age and both PGC-1? and PGC-1? and indicate that the MHC genes may to some extent be used to determine fibre-type composition in human skeletal muscle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1096-7206
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
103
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-81
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:21470888-Adult, pubmed-meshheading:21470888-Age Factors, pubmed-meshheading:21470888-Aged, pubmed-meshheading:21470888-Blood Glucose, pubmed-meshheading:21470888-Carrier Proteins, pubmed-meshheading:21470888-Diabetes Mellitus, Type 2, pubmed-meshheading:21470888-Female, pubmed-meshheading:21470888-Gene Expression Profiling, pubmed-meshheading:21470888-Gene Expression Regulation, pubmed-meshheading:21470888-Heat-Shock Proteins, pubmed-meshheading:21470888-Humans, pubmed-meshheading:21470888-Insulin, pubmed-meshheading:21470888-Male, pubmed-meshheading:21470888-Middle Aged, pubmed-meshheading:21470888-Muscle, Skeletal, pubmed-meshheading:21470888-Muscle Fibers, Skeletal, pubmed-meshheading:21470888-Myosin Heavy Chains, pubmed-meshheading:21470888-Polymorphism, Single Nucleotide, pubmed-meshheading:21470888-RNA, Messenger, pubmed-meshheading:21470888-Transcription Factors, pubmed-meshheading:21470888-Twins
pubmed:year
2011
pubmed:articleTitle
The expression of myosin heavy chain (MHC) genes in human skeletal muscle is related to metabolic characteristics involved in the pathogenesis of type 2 diabetes.
pubmed:affiliation
Lund University, Department of Clinical Sciences Malmö, Lund University Diabetes Centre, Scania University Hospital, Malmö, Sweden. anders_h.olsson@med.lu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't