Linked Life Data

21467749

Source:http://linkedlifedata.com/resource/pubmed/id/21467749

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-4-6
pubmed:abstractText
Our previous studies have demonstrated that maternal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes a reduction in gonadotropin biosynthesis in the fetal pituitary, resulting in the attenuated expression of steroidogenic proteins in the fetal gonads and the impairment of sexual behaviors in adulthood. However, the mechanism of the attenuation remains unknown. To address this issue, we investigated whether TCDD affects the pituitary production of gonadotropins, using cultured pituitary. In the absence of gonadotropin-releasing hormone (GnRH), a regulator of gonadotropin biosynthesis, TCDD did not affect the expression of gonadotropin mRNAs both in fetal and postnatal pituitaries. On the other hand, in the presence of GnRH, TCDD interfered with the synthesis of gonadotropin ?-subunit mRNAs only in the fetal pituitary. A protein kinase C (PKC) activator (phorbol 12-myristate 13-acetate) and a PKA activator (8-bromoadenosine-3' 5'-cyclic monophosphate) induced the expression of gonadotropin mRNAs in the fetal pituitary. Among the subunits, only the induction of ?-subunit was reduced by TCDD treatment. These results suggest that TCDD reduces gonadotropin biosynthesis via damage to GnRH-stimulated PKC and PKA signaling in a ?-subunit- and fetal age-specific manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1880-3989
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
221-9
pubmed:dateRevised
2011-7-21
pubmed:meshHeading
pubmed-meshheading:21467749-8-Bromo Cyclic Adenosine Monophosphate, pubmed-meshheading:21467749-Animals, pubmed-meshheading:21467749-Animals, Newborn, pubmed-meshheading:21467749-Environmental Pollutants, pubmed-meshheading:21467749-Female, pubmed-meshheading:21467749-Fetus, pubmed-meshheading:21467749-Gene Expression Regulation, Developmental, pubmed-meshheading:21467749-Gestational Age, pubmed-meshheading:21467749-Gonadotropins, Pituitary, pubmed-meshheading:21467749-Male, pubmed-meshheading:21467749-Organ Culture Techniques, pubmed-meshheading:21467749-Pituitary Gland, pubmed-meshheading:21467749-RNA, Messenger, pubmed-meshheading:21467749-Rats, pubmed-meshheading:21467749-Rats, Wistar, pubmed-meshheading:21467749-Tetrachlorodibenzodioxin, pubmed-meshheading:21467749-Tetradecanoylphorbol Acetate
pubmed:year
2011
pubmed:articleTitle
2,3,7,8-tetrachlorodibenzo-p-dioxin potentially attenuates the gene expression of pituitary gonadotropin ?-subunits in a fetal age-specific fashion: a comparative study using cultured pituitaries.
pubmed:affiliation
Graduate School of Pharmaceutical Sciences, Kyushu University, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't