Linked Life Data

21466289

Source:http://linkedlifedata.com/resource/pubmed/id/21466289

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-4-6
pubmed:abstractText
From our previous proteomic research, we found that translationally controlled tumor protein (TCTP) might play at least a partial role in colon adenocarcinoma progression. However, the precise impact of TCTP on colorectal cancer metastasis progression is currently still unknown. Therefore, immunology reagents are urgently needed to proceed with the next mechanism-related research. Moreover, the identification of TCTP expression level in tissue of colorectal cancer patients also requires substantial amounts of immunology reagents. In this report, monoclonal antibodies (MAbs) against to TCTP were made from hyperimmune Balb/c mice, by injecting 50??g of purified antigen intraperitoneally. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using purified protein. Finally six mouse hybridomas producing MAbs to TCTP were established. The MAbs obtained were fully characterized using Western blot analysis and immunohistochemistry. The results showed that these antibodies could be used for the preliminary application of the next mechanism-related research and TCTP expression level analysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1557-8348
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-5
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Preparation and characterization of monoclonal antibody against translationally controlled tumor protein.
pubmed:affiliation
The Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, North Guangzhou Road, Baiyun, Guangzhou, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't