Linked Life Data

2146494

Source:http://linkedlifedata.com/resource/pubmed/id/2146494

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-12-7
pubmed:databankReference
pubmed:abstractText
The protein sequence deduced from the open reading frame of a human placental cDNA encoding a cAMP-responsive enhancer (CRE)-binding protein (CREB-327) has structural features characteristic of several other transcriptional transactivator proteins including jun, fos, C/EBP, myc, and CRE-BP1. Results of Southwestern analysis of nuclear extracts from several different cell lines show that there are multiple CRE-binding proteins, which vary in size in cell lines derived from different tissues and animal species. To examine the molecular diversity of CREB-327 and related proteins at the nucleic acid level, we used labeled cDNAs from human placenta that encode two different CRE-binding proteins (CREB-327 and CRE-BP1) to probe Northern and Southern blots. Both probes hybridized to multiple fragments on Southern blots of genomic DNA from various species. Alternatively, when a human placental c-jun probe was hybridized to the same blot, a single fragment was detected in most cases, consistent with the intronless nature of the human c-jun gene. The CREB-327 probe hybridized to multiple mRNAs, derived from human placenta, ranging in size from 2-9 kilobases. In contrast, the CRE-BP1 probe identified a single 4-kilobase mRNA. Sequence analyses of several overlapping human genomic cosmid clones containing CREB-327 sequences in conjunction with polymerase chain reaction indicates that the CREB-327/341 cDNAs are composed of at least eight or nine exons, and analyses of human placental cDNAs provide direct evidence for at least one alternatively spliced exon. Analyses of mouse/hamster-human hybridoma DNAs by Southern blotting and polymerase chain reaction localizes the CREB-327/341 gene to human chromosome 2. The results indicate that there is a dichotomy of CREB-like proteins, those that are related by overall structure and DNA-binding specificity as well as those that are related by close similarities of primary sequences.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
920-30
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2146494-Amino Acid Sequence, pubmed-meshheading:2146494-Animals, pubmed-meshheading:2146494-Base Sequence, pubmed-meshheading:2146494-Chromosome Mapping, pubmed-meshheading:2146494-Chromosomes, Human, Pair 2, pubmed-meshheading:2146494-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:2146494-DNA, pubmed-meshheading:2146494-DNA-Binding Proteins, pubmed-meshheading:2146494-Exons, pubmed-meshheading:2146494-Female, pubmed-meshheading:2146494-Genetic Variation, pubmed-meshheading:2146494-Humans, pubmed-meshheading:2146494-Molecular Sequence Data, pubmed-meshheading:2146494-Peptide Fragments, pubmed-meshheading:2146494-Placenta, pubmed-meshheading:2146494-Pregnancy, pubmed-meshheading:2146494-RNA, Messenger, pubmed-meshheading:2146494-RNA Splicing, pubmed-meshheading:2146494-Rats, pubmed-meshheading:2146494-Transcription, Genetic
pubmed:year
1990
pubmed:articleTitle
Multiple adenosine 3',5'-cyclic [corrected] monophosphate response element DNA-binding proteins generated by gene diversification and alternative exon splicing.
pubmed:affiliation
Massachusetts General Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston 02114.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't