Source:http://linkedlifedata.com/resource/pubmed/id/21464822
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2011-6-29
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pubmed:abstractText |
Accumulating evidence indicates that mitochondria have a key role in non-alcoholic fatty liver disease (NAFLD). C57BL/6J mice were fed a choline-deficient, ethionine-supplemented (CDE) diet. Histological studies demonstrated accumulation of fat vacuoles in up to 90% of hepatocytes in mice fed the CDE diet for 14 days. In addition, a decrease in mitochondrial levels, together with an increase in superoxide radicals' levels were observed, indicating elevation of oxidative stress in hepatocytes. ATP levels were decreased in livers from CDE-fed mice after overnight fasting. This was accompanied by a compensative and significant increase in peroxisome-proliferator-activated receptor-? coactivator 1? (PGC1?) mRNA levels in comparison to control livers. However, there was a reduction in PGC1? protein levels in CDE-treated mice. Moreover, the expression of mitochondrial biogenesis genes nuclear respiratory factor 1 (NRF-1), mitochondrial transcription factor A (TFAM), mitochondrial transcription factor B1 (TFB1M) and mitochondrial transcription factor B2 (TFB2M), which are all regulated by PGC1? activity, remained unchanged in fasted CDE-treated mice. These results indicate impaired activity of PGC1?. The impaired activity was further confirmed by chromatin immunoprecipitation analysis, which demonstrated decreased interaction of PGC1? with promoters containing NRF-1 and NRF-2 response elements in mice fed the CDE diet. A decrease in PGC1? ability to activate the expression of the gluconeogenic gene phosphoenol-pyruvate carboxykinase was also observed. This study demonstrates, for the first time, that attenuated mitochondrial biogenesis in steatotic livers is associated with impaired biological activity of PGC1?.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/Ethionine,
http://linkedlifedata.com/resource/pubmed/chemical/Ppargc1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1530-0307
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1018-28
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pubmed:meshHeading |
pubmed-meshheading:21464822-Adenosine Triphosphate,
pubmed-meshheading:21464822-Animals,
pubmed-meshheading:21464822-Choline,
pubmed-meshheading:21464822-Chromatin Immunoprecipitation,
pubmed-meshheading:21464822-Diet,
pubmed-meshheading:21464822-Ethionine,
pubmed-meshheading:21464822-Fatty Liver,
pubmed-meshheading:21464822-Male,
pubmed-meshheading:21464822-Mice,
pubmed-meshheading:21464822-Mice, Inbred C57BL,
pubmed-meshheading:21464822-Mitochondria, Liver,
pubmed-meshheading:21464822-Oxidative Stress,
pubmed-meshheading:21464822-Polymerase Chain Reaction,
pubmed-meshheading:21464822-Reactive Oxygen Species,
pubmed-meshheading:21464822-Trans-Activators
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pubmed:year |
2011
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pubmed:articleTitle |
Fatty liver is associated with impaired activity of PPAR?-coactivator 1? (PGC1?) and mitochondrial biogenesis in mice.
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pubmed:affiliation |
The School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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