Linked Life Data

21464475

Source:http://linkedlifedata.com/resource/pubmed/id/21464475

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-6-2
pubmed:databankReference
pubmed:abstractText
Although calcium (Ca) concentration in cellular compartments has been suggested to be tightly regulated, Ca deficiency disorders such as blossom-end rot (BER) in tomato (Solanum lycopersicum) fruit may be induced by abnormal regulation of Ca partitioning and distribution in the cell. The objectives of this work were to analyze the effects of high expression of the constitutively functional Arabidopsis (Arabidopsis thaliana) Ca(2+)/H(+) exchanger (sCAX1) tonoplast protein in tomato fruit on cellular Ca partitioning and distribution, membrane integrity, and the transcriptional profile of genes potentially involved in BER development. Wild-type and sCAX1-expressing tomato plants were grown in a greenhouse. Wild-type plants did not develop BER, whereas sCAX1-expressing plants reached 100% BER incidence at 15 d after pollination. The sCAX1-expressing fruit pericarp had higher total tissue and water-soluble Ca concentrations, lower apoplastic and cytosolic Ca concentrations, higher membrane leakage, and Ca accumulation in the vacuole of sCAX1-expressing cells. Microarray analysis of healthy sCAX1-expressing fruit tissue indicated down-regulation of genes potentially involved in BER development, such as genes involved in membrane structure and repair and cytoskeleton metabolism, as well as up-regulation of genes that may have limited BER damage expansion, such as genes coding for heat shock proteins, glutathione S-transferases, and peroxidases. The results indicate that the high expression of the sCAX1 gene reduces cytosolic and apoplastic Ca concentrations, affecting plasma membrane structure and leading to BER symptom development in the fruit tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1532-2548
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
156
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
844-55
pubmed:dateRevised
2011-9-30
pubmed:meshHeading
pubmed-meshheading:21464475-Antiporters, pubmed-meshheading:21464475-Arabidopsis, pubmed-meshheading:21464475-Calcium, pubmed-meshheading:21464475-Cation Transport Proteins, pubmed-meshheading:21464475-Cell Membrane, pubmed-meshheading:21464475-Flowers, pubmed-meshheading:21464475-Fruit, pubmed-meshheading:21464475-Gene Expression Regulation, Plant, pubmed-meshheading:21464475-Imaging, Three-Dimensional, pubmed-meshheading:21464475-Lycopersicon esculentum, pubmed-meshheading:21464475-Models, Biological, pubmed-meshheading:21464475-Molecular Sequence Data, pubmed-meshheading:21464475-Phenotype, pubmed-meshheading:21464475-Plant Diseases, pubmed-meshheading:21464475-Plant Epidermis, pubmed-meshheading:21464475-Plants, Genetically Modified, pubmed-meshheading:21464475-Transcription, Genetic, pubmed-meshheading:21464475-Vacuoles
pubmed:year
2011
pubmed:articleTitle
Dynamic alternations in cellular and molecular components during blossom-end rot development in tomatoes expressing sCAX1, a constitutively active Ca2+/H+ antiporter from Arabidopsis.
pubmed:affiliation
Department of Plant Sciences, University of California, Davis, California 95616, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't