Linked Life Data

21462164

Source:http://linkedlifedata.com/resource/pubmed/id/21462164

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-4-4
pubmed:abstractText
Macrophages play key roles in wound repair and fibrosis by regulating extracellular matrix turnover. Macrophages can process matrix components themselves, but also recruit and alter the functions of other cell types that directly build or degrade extracellular matrix. Classically activated macrophages (CAM, also called M1) tend to promote tissue injury while alternatively activated macrophages (AAM, also called M2) are often linked with the mechanisms of wound repair and fibrosis. However, rather than promoting collagen deposition, recent studies suggest that arginase-1-expressing AAM suppress chronic inflammation and fibrosis by inhibiting antigen-specific T cell responses. This unit describes methods to measure arginase activity in macrophages and whole tissues as well as assays to quantify the T cell suppressive activity of AAMs. Modified hydroxyproline and soluble collagen assays that can be used to quantify collagen levels in tissues and brochoalveolar lavage fluid are also described. The protocols in this unit should provide the investigator with all the necessary information required to measure arginase activity and to correlate the observed activity with the progression and resolution of fibrosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1934-368X
pubmed:author
pubmed:copyrightInfo
© 2011 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit14.22
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Quantitative assessment of macrophage functions in repair and fibrosis.
pubmed:affiliation
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
pubmed:publicationType
Journal Article