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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1990-11-16
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pubmed:abstractText |
In an attempt to isolate gamma crystallin genes from Xenopus genomic library, we have obtained seventeen clones which strongly hybridized to the gamma 1 cDNA probe from Rana temporaria. Of these four clones are characterized. A restriction map was derived by using single and multiple digests with a variety of enzymes. Hybridization selection of lens mRNAs and in vitro translation produced a protein of 26,000 daltons. These data suggest that the above clones may contain at least part of the gamma or beta crystallin sequences.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1333-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2145835-Animals,
pubmed-meshheading:2145835-Bacteriophage lambda,
pubmed-meshheading:2145835-Blotting, Southern,
pubmed-meshheading:2145835-Cloning, Molecular,
pubmed-meshheading:2145835-Crystallins,
pubmed-meshheading:2145835-DNA,
pubmed-meshheading:2145835-Lens, Crystalline,
pubmed-meshheading:2145835-Molecular Weight,
pubmed-meshheading:2145835-Multigene Family,
pubmed-meshheading:2145835-Nucleic Acid Hybridization,
pubmed-meshheading:2145835-RNA, Messenger,
pubmed-meshheading:2145835-Rana temporaria,
pubmed-meshheading:2145835-Restriction Mapping,
pubmed-meshheading:2145835-Xenopus laevis
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pubmed:year |
1990
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pubmed:articleTitle |
Isolation and characterization of a distantly related member of the beta-gamma crystallin super gene family from Xenopus.
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pubmed:affiliation |
Eye Research Institute, Oakland University, Rochester, Michigan 48309.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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