Linked Life Data

21455970

Source:http://linkedlifedata.com/resource/pubmed/id/21455970

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-4-1
pubmed:abstractText
The ability to study proteins and protein interactions inside cells and tissues is important for elucidating how cells function in health and disease. The in situ proximity ligation assay (in situ PLA) presented here can be used to visualize proteins, protein-protein interactions, and post-translational modifications in cells and tissues. The method is based upon the use of antibodies that target the proteins involved in an interaction; hence, the method has the advantage that it can be used in clinical specimens, providing localized, quantifiable single molecule detection in single cells. This unit describes how in situ PLA can be used with fluorescence microscopy and flow cytometry to study proteins (obtaining high sensitivity and specificity of detection) and protein interactions. It also includes information on expected results and information on how to troubleshoot the assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1934-9300
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 9.36
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
In situ proximity ligation assay for microscopy and flow cytometry.
pubmed:affiliation
Department of Immunology, Uppsala University, Uppsala, Sweden.
pubmed:publicationType
Journal Article