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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2011-5-18
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pubmed:abstractText |
A single nucleotide polymorphism (SNP) is a common genetic variation when a single nucleotide differs between members of a species or paired chromosome. Due to its association with disease susceptibility and drug resistance, SNP detection is of great value in studying the variation in drug responses. Here we present two quantitative SNP detection methods for a single-base mismatch in RNA, based on nick-joining and nick-generating activities of T4 RNA ligase and DNAzyme, respectively. T4 RNA ligase successfully discriminated a one-base mismatch in the ligation junction, and the designed DNAzyme cleaved RNA by discerning a single-base mismatch in the cleaving site.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1096-0309
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
414
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
303-5
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pubmed:meshHeading |
pubmed-meshheading:21453671-Base Pair Mismatch,
pubmed-meshheading:21453671-DNA, Catalytic,
pubmed-meshheading:21453671-Electrophoresis, Agar Gel,
pubmed-meshheading:21453671-Molecular Probe Techniques,
pubmed-meshheading:21453671-Oligonucleotide Probes,
pubmed-meshheading:21453671-Polymorphism, Single Nucleotide,
pubmed-meshheading:21453671-RNA,
pubmed-meshheading:21453671-RNA Ligase (ATP),
pubmed-meshheading:21453671-Viral Proteins
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pubmed:year |
2011
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pubmed:articleTitle |
Detection of single-base mutation in RNA using T4 RNA ligase-based nick-joining or DNAzyme-based nick-generation.
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pubmed:affiliation |
Department of Bioscience and Biotechnology, and WCU and BRL Program, Konkuk University, Seoul, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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