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rdf:type | |
lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0022688,
umls-concept:C0039194,
umls-concept:C0086418,
umls-concept:C0108779,
umls-concept:C0162801,
umls-concept:C0205280,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1704788,
umls-concept:C1711351,
umls-concept:C1719914,
umls-concept:C2828406
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pubmed:issue |
8
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pubmed:dateCreated |
1990-11-2
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pubmed:abstractText |
In order to characterize the CD3 zeta-related protein found in human natural killer (NK) cells and compare it with CD3 zeta expressed in T lymphocytes, the present study was performed. A polyclonal CD3-CD16+NK population displaying a strong non-major histocompatibility complex-restricted cytotoxic activity against the NK target K-562 was isolated and a product corresponding to CD3 zeta amplified using the polymerase chain reaction method. This 0.6-kb product was present in similar amounts in NK cells and T cells. In contrast, a product corresponding to CD3 delta was amplified from T lymphocytes exclusively. Thus, the CD3 zeta product detected in NK cells did not originate from contaminating T cells. DNA sequence analysis of two independent polymerase chain reaction products from the NK cells demonstrates that human NK cells and mature T cells share a CD3 zeta subunit with an identical primary amino acid sequence. The nucleotide sequence of a third NK-derived cDNA revealed an insertion of a CAG triplet encoding an additional glutamine residue in the cytoplasmic domain. Since this residue is encoded by nucleotides at a putative RNA splice junction, it possibly results from a difference in pre-mRNA splicing. Taken together, these data show that CD3 zeta is not structurally distinct in NK cells and in T lymphocytes.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1741-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2145165-Amino Acid Sequence,
pubmed-meshheading:2145165-Antigens, CD3,
pubmed-meshheading:2145165-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2145165-Base Sequence,
pubmed-meshheading:2145165-Chromosome Mapping,
pubmed-meshheading:2145165-Cloning, Molecular,
pubmed-meshheading:2145165-DNA,
pubmed-meshheading:2145165-Gene Expression,
pubmed-meshheading:2145165-Humans,
pubmed-meshheading:2145165-Killer Cells, Natural,
pubmed-meshheading:2145165-Molecular Sequence Data,
pubmed-meshheading:2145165-Polymerase Chain Reaction,
pubmed-meshheading:2145165-Receptors, Antigen, T-Cell,
pubmed-meshheading:2145165-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2145165-T-Lymphocytes,
pubmed-meshheading:2145165-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Human natural killer cells and mature T lymphocytes express identical CD3 zeta subunits as defined by cDNA cloning and sequence analysis.
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pubmed:affiliation |
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA 02115.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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