Source:http://linkedlifedata.com/resource/pubmed/id/21451544
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2011-6-15
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pubmed:abstractText |
Estrogen is an important regulator of dermal fibroblast functions, including extracellular matrix (ECM) synthesis. Estrogen mediates its effects through estrogen receptors (ERs), ER? and ER?; however, regulation of ERs in dermal fibroblasts remains poorly understood. Friend leukemia integration factor 1 (Fli1), a member of the Ets transcription factor family, has been shown to play a pivotal role in regulation of the ECM genes in dermal fibroblasts. The aim of this study was to examine a possible interaction between Fli1 and estrogen pathways, focusing on ER?. We show that treatment of human dermal fibroblasts with transforming growth factor-? (TGF-?) increases ER? protein and mRNA levels. Similarly, ER? expression was increased in response to small interfering RNA (siRNA)-mediated depletion of Fli1, suggesting that Fli1 is a mediator of the TGF-? effects on ER? expression. Accordingly, we showed that Fli1 binds to the most proximal region of the ER? promoter, and dissociates from the promoter upon TGF-? treatment. An inverse correlation between Fli1 and ER? expression levels was confirmed in cultured skin fibroblasts obtained from Fli1(+/-) mice and in the skin of Fli1(+/-) mice in vivo. This study supports a role of Fli1 as a negative regulator of the ER? gene in dermal fibroblasts.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Receptor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/FLI1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Protein c-fli-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/estrogen receptor alpha, human
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1523-1747
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
131
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1469-76
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pubmed:meshHeading |
pubmed-meshheading:21451544-Cells, Cultured,
pubmed-meshheading:21451544-Estrogen Receptor alpha,
pubmed-meshheading:21451544-Extracellular Matrix,
pubmed-meshheading:21451544-Fibroblasts,
pubmed-meshheading:21451544-Gene Expression Regulation,
pubmed-meshheading:21451544-Humans,
pubmed-meshheading:21451544-Promoter Regions, Genetic,
pubmed-meshheading:21451544-Proto-Oncogene Protein c-fli-1,
pubmed-meshheading:21451544-Skin,
pubmed-meshheading:21451544-Transforming Growth Factor beta
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pubmed:year |
2011
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pubmed:articleTitle |
Fli1 is a negative regulator of estrogen receptor ? in dermal fibroblasts.
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pubmed:affiliation |
Arthritis Center, Boston University School of Medicine, Boston, Massachusetts, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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