Linked Life Data

21451544

Source:http://linkedlifedata.com/resource/pubmed/id/21451544

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-6-15
pubmed:abstractText
Estrogen is an important regulator of dermal fibroblast functions, including extracellular matrix (ECM) synthesis. Estrogen mediates its effects through estrogen receptors (ERs), ER? and ER?; however, regulation of ERs in dermal fibroblasts remains poorly understood. Friend leukemia integration factor 1 (Fli1), a member of the Ets transcription factor family, has been shown to play a pivotal role in regulation of the ECM genes in dermal fibroblasts. The aim of this study was to examine a possible interaction between Fli1 and estrogen pathways, focusing on ER?. We show that treatment of human dermal fibroblasts with transforming growth factor-? (TGF-?) increases ER? protein and mRNA levels. Similarly, ER? expression was increased in response to small interfering RNA (siRNA)-mediated depletion of Fli1, suggesting that Fli1 is a mediator of the TGF-? effects on ER? expression. Accordingly, we showed that Fli1 binds to the most proximal region of the ER? promoter, and dissociates from the promoter upon TGF-? treatment. An inverse correlation between Fli1 and ER? expression levels was confirmed in cultured skin fibroblasts obtained from Fli1(+/-) mice and in the skin of Fli1(+/-) mice in vivo. This study supports a role of Fli1 as a negative regulator of the ER? gene in dermal fibroblasts.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1523-1747
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1469-76
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Fli1 is a negative regulator of estrogen receptor ? in dermal fibroblasts.
pubmed:affiliation
Arthritis Center, Boston University School of Medicine, Boston, Massachusetts, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural