Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
2011-3-29
pubmed:abstractText
Tail-anchored (TA) proteins are post-translationally targeted to and inserted into the endoplasmic reticulum (ER) membrane through their single C-terminal transmembrane domain. Membrane insertion of TA proteins in mammalian cells is mediated by the ATPase TRC40/Asna1 (Get3 in yeast) and a receptor in the ER membrane. We have identified tryptophan-rich basic protein (WRB), also known as congenital heart disease protein 5 (CHD5), as the ER membrane receptor for TRC40/Asna1. WRB shows sequence similarity to Get1, a subunit of the membrane receptor complex for yeast Get3. Using biochemical and cell imaging approaches, we demonstrate that WRB is an ER-resident membrane protein that interacts with TRC40/Asna1 and recruits it to the ER membrane. We identify the coiled-coil domain of WRB as the binding site for TRC40/Asna1 and show that a soluble form of the coiled-coil domain interferes with TRC40/Asna1-mediated membrane insertion of TA proteins. The identification of WRB as a component of the TRC (Get) pathway for membrane insertion of TA proteins raises new questions concerning the proposed roles of WRB (CHD5) in congenital heart disease, and heart and eye development.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-10320398, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-11590105, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-11753368, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-12821639, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-14731773, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-14990965, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-1531166, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-15716491, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-16489338, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-17382883, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-17629691, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-18477612, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-18724936, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-19207181, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-19325107, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-19675567, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-19706470, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-19812306, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-19948960, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-20015340, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-20106980, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-20375064, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-20516149, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-20646998, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-20696390, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-3670292, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-7835332, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-9480850, http://linkedlifedata.com/resource/pubmed/commentcorrection/21444755-9544840
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1477-9137
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1301-7
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.
pubmed:affiliation
Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, INF282, 69120 Heidelberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't