Linked Life Data

21443546

Source:http://linkedlifedata.com/resource/pubmed/id/21443546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-7-4
pubmed:abstractText
High-level expression of foreign proteins in chloroplasts of transplastomic plants provides excellent opportunities for the development of oral vaccines against a range of debilitating or fatal diseases. The HIV-1 capsid protein p24 and a fusion of p24 with the negative regulatory protein Nef (p24-Nef) accumulate to ?4% and ?40% of the total soluble protein of leaves of transplastomic tobacco (Nicotiana tabacum L.) plants. This study has investigated the immunogenicity in mice of these two HIV-1 proteins, using cholera toxin B subunit as an adjuvant. Subcutaneous immunization with purified chloroplast-derived p24 elicited a strong antigen-specific serum IgG response, comparable to that produced by Escherichia coli-derived p24. Oral administration of a partially purified preparation of chloroplast-derived p24-Nef fusion protein, used as a booster after subcutaneous injection with either p24 or Nef, also elicited strong antigen-specific serum IgG responses. Both IgG1 and IgG2a subtypes, associated with cell-mediated Th1 and humoral Th2 responses, respectively, were found in sera after subcutaneous and oral administration. These results indicate that chloroplast-derived HIV-1 p24-Nef is a promising candidate as a component of a subunit vaccine delivered by oral boosting, after subcutaneous priming by injection of p24 and/or Nef.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1467-7652
pubmed:author
pubmed:copyrightInfo
© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.
pubmed:issnType
Electronic
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
629-38
pubmed:meshHeading
pubmed-meshheading:21443546-AIDS Vaccines, pubmed-meshheading:21443546-Adjuvants, Immunologic, pubmed-meshheading:21443546-Administration, Oral, pubmed-meshheading:21443546-Animals, pubmed-meshheading:21443546-Chloroplasts, pubmed-meshheading:21443546-Female, pubmed-meshheading:21443546-HIV Core Protein p24, pubmed-meshheading:21443546-Immunity, Humoral, pubmed-meshheading:21443546-Immunization, Secondary, pubmed-meshheading:21443546-Injections, Subcutaneous, pubmed-meshheading:21443546-Mice, pubmed-meshheading:21443546-Mice, Inbred BALB C, pubmed-meshheading:21443546-Mice, Inbred C57BL, pubmed-meshheading:21443546-Nicotine, pubmed-meshheading:21443546-Plants, Genetically Modified, pubmed-meshheading:21443546-Tobacco, pubmed-meshheading:21443546-nef Gene Products, Human Immunodeficiency Virus
pubmed:year
2011
pubmed:articleTitle
Immunogenicity of chloroplast-derived HIV-1 p24 and a p24-Nef fusion protein following subcutaneous and oral administration in mice.
pubmed:affiliation
Department of Plant Sciences, University of Cambridge, Cambridge, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't