Source:http://linkedlifedata.com/resource/pubmed/id/21440432
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2011-6-7
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pubmed:abstractText |
This article summarizes general design principles for functional metagenomics. The focus is on Escherichia coli as an expression host, although alternative host-vector systems are discussed in relation to optimizing gene recovery in activity-based screens. Examples of DNA isolation and enrichment approaches, library construction and phenotypic read-out are described with special emphasis on the use of high throughput technologies for rapid isolation of environmental clones encoding phenotypic traits of interest.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1879-0429
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pubmed:author | |
pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
465-72
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pubmed:meshHeading |
pubmed-meshheading:21440432-DNA, Bacterial,
pubmed-meshheading:21440432-Escherichia coli,
pubmed-meshheading:21440432-Gene Library,
pubmed-meshheading:21440432-Genes, Bacterial,
pubmed-meshheading:21440432-Genetic Vectors,
pubmed-meshheading:21440432-Metagenomics,
pubmed-meshheading:21440432-Microbial Consortia,
pubmed-meshheading:21440432-Sequence Analysis, DNA
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pubmed:year |
2011
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pubmed:articleTitle |
The art and design of functional metagenomic screens.
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pubmed:affiliation |
Department of Microbiology & Immunology, University of British Columbia, Canada.
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pubmed:publicationType |
Journal Article
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