|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
1990-9-14
|
|
pubmed:abstractText |
The length of amino acid sequence at the NS1-NS2A juncture of dengue virus that is required for specific cleavage effected by the cis-acting function of NS2A was identified by deletion analysis. Recombinant DNA sequences of NS1-NS2A, each containing a deletion in NS1 followed by a sequence of 3 to 20 amino acids at the C terminus of NS1 preceding the cleavage site, were constructed and expressed with vaccinia virus as a vector. The NS1 product of recombinant vaccinia virus-infected cells was immunoprecipitated and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The occurrence of cleavage between NS1 and NS2A was indicated by the appearance of shortened NS1. Failure to cleave this site yielded a large NS1-NS2A fusion protein. This analysis indicated that a minimum length of eight amino acids at the NS1 C terminus preceding the NS1-NS2A juncture is required for cleavage to take place. Comparison of this eight-amino-acid sequence of the NS1 C terminus of dengue type 4 virus with the analogous sequences of 12 other flaviviruses suggests that the consensus cleavage site sequence is as follows: (table; see text)
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2136778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2501515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2522997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2554575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2674479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2741348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2826659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2826667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2827375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2952760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2992152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-2998002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3009829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3011278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3022479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3027980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3039728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3052288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3286889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3316711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3388770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3672932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3686827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3753811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3803928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3855247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-3939316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-4023707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143546-4032478
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
64
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4573-7
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2143546-Amino Acid Sequence,
pubmed-meshheading:2143546-Base Sequence,
pubmed-meshheading:2143546-Capsid,
pubmed-meshheading:2143546-Chromosome Deletion,
pubmed-meshheading:2143546-Cloning, Molecular,
pubmed-meshheading:2143546-Dengue Virus,
pubmed-meshheading:2143546-Genes, Viral,
pubmed-meshheading:2143546-Glycoside Hydrolases,
pubmed-meshheading:2143546-Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase,
pubmed-meshheading:2143546-Molecular Sequence Data,
pubmed-meshheading:2143546-Oligonucleotide Probes,
pubmed-meshheading:2143546-Peptide Hydrolases,
pubmed-meshheading:2143546-Recombinant Fusion Proteins,
pubmed-meshheading:2143546-Substrate Specificity,
pubmed-meshheading:2143546-Vaccinia virus,
pubmed-meshheading:2143546-Viral Core Proteins,
pubmed-meshheading:2143546-Viral Nonstructural Proteins
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Cleavage of dengue virus NS1-NS2A requires an octapeptide sequence at the C terminus of NS1.
|
|
pubmed:affiliation |
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
|
|
pubmed:publicationType |
Journal Article
|
